FV, FVIII and Fibrinogen Activity in Fresh Frozen Plasma, Frozen Plasma and Cryoprecipitate: Observational Cross-sectional Study

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Ibrahim Youssef
Soha Abuelela
Walaa Hamza


Objective: Studies proved that storing whole blood overnight at 4°C resulted in a decrease in the activity of coagulation factor FVIII, without significant loss of activity of coagulation factors FV or fibrinogen. This study is conducted to compare the activity of labile factors V and VIII as well as fibrinogen level in FFP with that of FP24 and to assess their levels in cryoprecipitate and cryosupernatant bags as well.

Materials and Methods: FFP bags separated from whole blood within 8 hours were compared to FP24 bags separated within 24 hours, cryoprecipitate and cryosupernatant after phlebotomy in terms of coagulation factors V and VIII activity and level of fibrinogen by standard methods using (automated coagulometer STA Compact, Stago, France).

Results: A statistically significant loss of factor VIII activity in FP24 compared to FFP was detected; while, the fall in activity of factor V and fibrinogen level was not statistically significant. A highly statistically significant difference was elicited regarding factor VIII, factor V and fibrinogen when comparing cryoprecipitate and cryosupernatant samples. On comparing FFP and cryoprecipitate regarding factor VIII, factor V and fibrinogen a highly statistically significant difference was elicited.

Conclusion: The retention in factor activities, in particular factor V, in FP, indicates the lack of relevant adverse changes when extending the hold period for plasma units. The reduction in factor VIII activity doesn’t reduce the quality of FP.

Factor V, factor VIII, fibrinogen, fresh frozen plasma, plasma frozen within 24 h, cryoprecipitate

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How to Cite
Youssef, I., Abuelela, S., & Hamza, W. (2019). FV, FVIII and Fibrinogen Activity in Fresh Frozen Plasma, Frozen Plasma and Cryoprecipitate: Observational Cross-sectional Study. Asian Hematology Research Journal, 2(2), 1-7. Retrieved from http://journalahrj.com/index.php/AHRJ/article/view/30106
Original Research Article


Stanworth SJ, Tinmouth AT. Plasma transfusion and use of albumin. In: Simon TL, editor. Rossi’s principles of transfusion medicine. 4th ed. Oxford, West Sussex, New Jersey: Blackwell Publishing. 2009; 287-297.

Franchini M, Lippi G. Fibrinogen replacement therapy: a critical review of the literature. Blood Transfusion. 2012;10 (1):23-27.

Alhumaidan H, Cheves T, Holme S, Sweeney J. Stability of coagulation factors in plasma prepared after a 24-hour room temperature hold. Transfusion. 2010;50 (9):1934-1942.

Cardigan R, Van der Meer PF, Pergande C, Cookson P, Baumann-Baretti B, Cancelas JA. Coagulation factor content of plasma produced from whole blood stored for 24 hours at ambient temperature: Results from an international multicenter BEST Collaborative study. 2011;51(1): 50S-7.

AABB. Technical Manual, 18th edition. Maryland; 2014.

Dogra M, Sidhu M, Vasudev R, Dogra A. Comparative analysis of activity of coagulation factors V and VIII and level of fibrinogen in fresh frozen plasma and frozen plasma. Asian Journal of Transfusion Science. 2015;9(1): 6-8.

Agus N, Yilmaz N, Colak A, Liv F. Levels of factor VIII and factor IX in fresh frozen plasma produced from whole blood stored at 4ºC overnight in Turkey. 2012. Blood Transfusion. 2012;10(2):191-193.

Alakech B, Miller B, Berry TH, Ambruso DR. Coagulation profile for cryoprecipitate produced from 24 hour stored whole blood. Lab Med. 2009;40:540-543.

Sheffield WP, Bhakta V, Jenkins C, Devine DV. Conversion to the buffy coat method and quality of frozen plasma derived from whole blood donations in Canada. Transfusion. 2010;50(5):1043-1049.

Liumbruno G, Bennardello F, Lattanzio A, Piccoli P, Rosetti G. Recommendations for the transfusion of plasma and platelets. Blood Transfus. 2009;7(2):132-150.

Stanworth SJ. The evidence-based use of FFP and cryoprecipitate for abnormalities of coagulation tests and clinical coagulopathy. Hematology Am Soc; Hematol Educ Program. 2007;179-186.

Subramaniyan R, Marwaha N, Jain A, Ahluwalia J. Factors affecting the quality of cryoprecipitate. Asian Journal of Transfusion Science. 2017;11(1):33-39.

Omidkhoda A, Tabatabaei MR, Atarodi K, Karimi K. A comparative study of the effects of temperature, time and factor VIII assaytype on FVIII activity in cryoprecipitate in Iran. Blood Transfusion. 2011;9(4):394-399.

Chandler WL, Ferrell C, Lee J, Tun T, Kha H. Comparison of three methods for measuring factor VIII levels in plasma. Am J Clin Pathol. 2003;120(1):34-39.

Barrowcliffe TW, Raut S, Sands D, Hubbard AR. Coagulation and chromogenic assays of factor VIII activity: general aspects, standardization, and recommendations. Semin Thromb Hemost. 2002;28(3):247-256.