Haematological Response to One-month Varying Doses Treatment of Wistar Rats with Desmodium adscendens

Main Article Content

S. A. Seriki
I. K. Nyoro

Abstract

Increasing consumption of traditional medicinal plants to manage medical conditions in recent times is driven by the belief that herbal products are generally safe and efficient. This has been proven wrong as several patients have come down with side effects of some of these herbs in the course of, or after their use. Desmodium adscendens is one of these herbs notably used to manage medical conditions. The current study aimed at determining the haematological response to the use of this herb in low, median and high doses, with the aim to generate data (evidence) as per safety profile (ADR) and the use of Desmodium adscendens in humans. The study which was carried out using 24 Wistar rats lasted for one month. Low, median and high doses of aqueous leave extract of the herb were administered on three groups of rats (n=6). And the responses in these groups were compared to one another and also to a control group. Desmodium adscendens did not have any significant effect on Red blood cell (RBC) count, Haematocrit (PCV), Haemoglobin (Hb) concentration, Mean corpuscular volume (MCV), Mean corpuscular haemoglobin concentration (MCHC), Mean corpuscular haemoglobin (MCH), White blood cell (WBC) count; (Total WBC count, Differential WBC), Platelet count, Platelet indices (PDW, MPV, P-LCR, Plateletcrit). However, the extract caused a significant decrease in the Red-cell Distribution Width (RDW). The leaf extract of D. adscendens also significantly reduced total protein and globulin concentrations in the blood plasma, but caused a significant (p < 0.05) increase in plasma albumin concentration. Desmodium adscendens did not cause significant effect on RBC, WBC and PLT indices. Its use in treatment and management of disease conditions is therefore not likely to have any adverse effect on the body’s blood parameters.

Keywords:
Desmodium adscendens, anisocytosis, reticulocytes, dehydration, Red-cell Distribution Width (RDW)

Article Details

How to Cite
Seriki, S. A., & Nyoro, I. K. (2019). Haematological Response to One-month Varying Doses Treatment of Wistar Rats with Desmodium adscendens. Asian Hematology Research Journal, 2(2), 1-15. Retrieved from http://journalahrj.com/index.php/AHRJ/article/view/30107
Section
Original Research Article

References

Nergard CS, Diallo D, Inngjerdingen K, Michaelsen TE, Matsumoto T, Kiyohara H. Medicinal use of Cochlospermum tinctorium in mali anti-ulcer-, radical scavenging- and immunomodulating activities of polymers in the aqueous extract of the roots. Journal of Ethnopharmacology. 2005;96:255–69.

Lewington A. Plants for people: The Natural History Museum, Cromwell Road, London. 1990;135-167.

Principe P. The economic value of biological diversity among medicinal plants; organization for economic co-operation and development. Environmental Monograph, Paris. 1989;140.

De Smet PA. Health Risk of Herbal Remedies. Druf Saf. 1995;13(2):81–93.

Taylor L. The healing power of rainforest herbs. Square One Publishers: USA. 2005; 528.

Addy ME. Some Secondary Plant Metabolites in Desmodium adscendens and their effects on Anaihidonic Acid Metabolism. Prostaglandins Leukotrienes Essent. Fatty Acids. 1997;47(1):85-91.

Evans WC. Trease and evans pharmacognosy, 15th edition, W.B. Sanders London. 2002;214-393, 419.

Seriki SA, Odetola AO, Adebayo OF. Analysis of phytoconstituents of Desmodium adscendens in relation to its therapeutic properties. American Journal of Biomedical Sciences & Resources. 2019; (4). AJBSR.MS.ID.000598.
DOI: 10.34297/AJBSR.2019.02.000598

Adeneye AA, Olagunju JA, Elias SO, Olatunbosun DO, Mustafa AO, Adeshile OI, Ashaolu AO, Laoye TA, Bamigboye AO, Adeoye AO. Protective activities of the aqueous root extract of Harungana madagascariensis in acute and repeated acetaminophen hepatotoxic rats. International Journal of Applied Research in Natural Products. 2008;1(3): 29-42.

Kengni F, Tala DS, Djimeli MN, Fodouop SPC, Kodjio N, Magnifouet HN, Gatsing D. In vitro antimicrobial activity of Harungana madagascariensis and Euphorbia prostrata extracts against some pathogenic Salmonella sp. International Journal of Biological and Chemical Science. 2013; 7(3):1106-1118.

Ohwada K. Improvement of cardiac puncture in mice. Jikken Dobutsu. 1986; 3:353-355.

Guarin NG. Plantas medicinais do Estado do Mato Grosso. Associação Brasileira de Educação Agrícola Superior. 1996;31.

Ermst E. The efficacy of herbal medicine- an overview. Tundam Clinical Pharmacology. 2005;19:405-409.

MacManus OB. An activator of calcium-dependent potassium channels isolated from a medicinal herb. Biochemistry. 1993; 32(24):6128–33.

Asante-Poku S, Sasakibara J, Addy ME. Alkaloids of Desmodium adscendens. Ghana Medical Journal. 1988;22:111-115.

Barbara J. Bain. Blood cell morphology in health and disease. Dacie and Lewis Practical Haematology, 12th Edition. 2017; 61-92.

Cushnie TP, Cushnie B, Lamb AJ. Alkanoids: An overview of their antibacterial, anti-biotic enhancing and antivirulence activities. International Journal of Antimicrobial Agents. 2014; 44(5):377-386.

Takako Y, Eun JC, Takako N. Influence f green tea polyphenol in rats with argini-induced renal failure. Journal of Agricultural and Food Chemistry. 2003; 51(8):2421-2425.

Rajput ZI, Hu SH, Xiao CW, Arijo AG. Adjuvant effects of saponins on animal immune responses. J Zhejiang University Science B. 2007;8(3):153-161.