Asian Hematology Research Journal 2020-01-24T07:01:42+00:00 Asian Hematology Research Journal Open Journal Systems <p style="text-align: justify;"><strong>Asian Hematology Research Journal</strong>&nbsp;aims to publish&nbsp;high-quality&nbsp;papers (<a href="/index.php/AHRJ/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of&nbsp;‘Hematology research’. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer-reviewed, open access INTERNATIONAL journal.</p> Acute Monoblastic Leukemia (AML-M5) with Concomitant Hemophagocytic Lymphohistiocytosis: Presenting as Pancytopenia 2020-01-23T07:46:25+00:00 Sonal . Deepti Gupta Nupur Trivedi Shefali Agarwal Archana Agarwal Anjali Tewari Priyanka Verma <p>Hemophagocytic Lymphohistiocytosis (HLH) is an aggressive hyperinflammatory condition characterized by prolonged fever, cytopenias, hepatosplenomegaly and hemophagocytosis with a high rate of mortality. Its diagnosis is a challenge for pathologists as well as clinicians due to variable overlap of symptoms with other severe diseases. AML-associated hemophagocytic lymphohistiocytosis is a rare event, reported only in limited members.</p> <p>We, hereby report a case of 14 year old male child who came to our hematology department with a history of prolonged fever, pancytopenia and 40 transfusions in last one year. Peripheral blood counts showed 15% blastoid atypical cells. Bone marrow revealed acute leukaemia with evidence of haemophagocytosis. A diagnosis of AML-M5 with HLH was made after flow cytometry and completion of clinico-biochemical criteria for HLH.</p> <p>Patients with HLH in acute leukaemia may have low blast counts on peripjeral blood leading to much delay in the diagnosis. This case report provides helpful clinical experience related to diagnosis of this rare entity.</p> 2020-01-23T00:00:00+00:00 ##submission.copyrightStatement## Flow Cytometry Findings of Aberrant Expression in a Cohort of Patients with Acute Myeloid Leukaemia 2020-01-24T07:01:42+00:00 C. C. Kariyawasan B. L. T. Balasuriya S. A. C. D. Ranatunga <p><strong>Background: </strong>Acute leukaemia is defined as the presence of over 20% of blasts cells in the blood or bone marrow. Acute myeloid leukaemia (AML) and acute lymphoblastic leukaemia (ALL) are the 2 main types. AMLs have characteristic morphological findings and molecular features with different surface and cytoplasmic cluster of differentiation (CD) markers. These CD markers are determined by immunophenotyping/flow cytometry on leukocytes which helps with accurate diagnosis and reproducibility of AMLs. Flow cytometry plays an important role in the diagnosis, sub classification and monitoring of patients with AML. AML generally shows aberrant CD expression or co- expression in relation to normal myeloid cells.</p> <p><strong>Objective of the Study: </strong>Objective of the Study was to evaluate the frequency and the pattern of aberrant CD expression in AML patients referred to a tertiary care hospital in Sri Lanka in comparison to other published data. There was no comparative data available in respect of Sri Lanka.</p> <p><strong>Materials and Methods: </strong>A retrospective descriptive study including 26 cases of AML diagnosed over a period of 12 months were analyzed. Diagnosis of AML was made by morphology of peripheral blood, bone marrow, trephine biopsies, Sudan Black B stain and the immunophenotypic analysis by multiparameter flow cytometry on bone marrow aspirates or peripheral blood. The markers used in flow cytometry were CD 45, CD34, CD19, CD7, smCD3, cyCD3, cyMPO, cyCD79a, CD20, CD15, CD10, CD5, HLADR, CD64, CD13, CD117, CD33, CD14.</p> <p><strong>Results: </strong>Among the 26 AML patients, 15 cases (57.69%) had the conventional CD antigen expressions of myeloid lineage. Other 11 cases (42.3%) were AML with aberrant expression of CD markers. Aberrancies of cyCD3 and CD7 were observed in 54.5% and 45.4% AML cases, respectively. smCD3 in 1 case out of 11 aberrant AML cases. Co expression of T lymphoid markers with myeloid markers occurred in 23% cases in our study. CD13 was not expressed in 1 case out of 5 AML- M4 cases and 1 case out of 7 AML- M1.&nbsp; CD33 was not expressed in 1 case out of 2 AML -M0 cases.</p> <p><strong>Conclusion: </strong>We conclude that aberrant expression of CD markers is seen in a significant population of AMLs. cyCD 3, CD7 and smCD 3 were the aberrant markers present in our study population with cyCD3 showing highest frequency.</p> 2020-01-24T00:00:00+00:00 ##submission.copyrightStatement## Determination of Some Haemotological Parameters in Children Infected with Plasmodium falciparum in Murtala Muhammad Specialist Hospital, Kano State, Nigeria 2020-01-06T11:12:03+00:00 I. Abdulmumin E. O. Osime M. Abdullahi I. M. Zubair <p><strong>Aim: </strong>The aim of the study is to determination of Some Haemotological Parameters in children infected with<em> Plasmodium falciparum</em> in Murtala Muhammad Specialist Hospital, Kano, Kano State, North Western Nigeria.</p> <p><strong>Study Design: </strong>The study is analytic study.</p> <p><strong>Place and Duration of the Study: </strong>In this research of the 350 children between the aged groups of 1-10 years infected with<em> Plasmodium falciparum</em> attending Murtala Muhammad Specialist Hospital Kano State were used for the study.</p> <p><strong>Methodology:</strong> Malaria Rapid diagnostic kids (RDTs) kits were used for qualitative immunochromatographic flow tests in a dipstick (strip) or cassette forms that detect malaria antigen present in peripheral blood. Haemoglobin, Packed Cell Volume (PCV) and Reticulocytes count were determined using standard procedure.</p> <p><strong>Results: </strong>A total of 350 subjects with<em> Plasmodium falciparum, </em>190 (54.3%) male and 160 (45.7%) female with 100 control subjects both male and female were recruited in this study aged from 1 – 10 years. Relative distribution of 212(60.6%) and 138(53.8%) of the subjects were anaemic based on PCV and haemoglobin values respectively. It was also observed that 216(61.8%) with high reticulocytes and 134(38.2%) with normal value, while 0(0%) have lower value. However, the comparative mean value and standard deviation of female PCV, haemoglobin of <em>Plasmodium falciparum</em> patients and control were significantly reduced (P&gt;0.05), while reticulocytes of <em>Plasmodium falciparum</em> patients and control were significantly high (P&lt;0.05).</p> <p><strong>Conclusion: </strong>In conclusion, the result indicated low PCV and haemoglobin among of male and female infected with <em>Plasmodium falciparum</em> because were significantly reduced (P&gt;0.05) in patients and control, while reticulocytes of <em>Plasmodium falciparum</em> patients and control were significantly high (P&lt;0.05).</p> 2020-01-06T00:00:00+00:00 ##submission.copyrightStatement##