Asian Hematology Research Journal <p style="text-align: justify;"><strong>Asian Hematology Research Journal</strong>&nbsp;aims to publish&nbsp;high-quality&nbsp;papers (<a href="/index.php/AHRJ/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of&nbsp;‘Hematology research’. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer-reviewed, open access INTERNATIONAL journal.</p> en-US (Asian Hematology Research Journal) (Asian Hematology Research Journal) Fri, 06 Mar 2020 11:09:07 +0000 OJS 60 Primary Plasma Cell Leukaemia in a Young HIV Infected Male, Associated with Epstein-barr Virus <p><strong>Background:</strong> Primary Plasma Cell Leukaemia (PPCL) in young patients has been evaluated in very few studies in the English literature. The aim of this study is to describe a case of PPCL in a young HIV infected male associated with EBV. This is first such case in Indian literature. This is an interesting study that reported a rare case where primary plasma cell leukaemia is observed in a young HIV infected male, which will be valuable case for future studies. Thorough clinical examinations were done and reported in detail.</p> <p><strong>Methods:</strong> A 35 years old male presented with abdominal pain, backache and asthenia. A thorough workup was done including clinical examination, CBP, KFT, LFT, serology (HIV, HBsAg), Serum electrophoresis, radiology and bone marrow examination. A systematic literature review was conducted by searching the Pub Med and National Centre for Biotechnology Information database.</p> <p><strong>Results:</strong> Patient had hepatosplenomegaly and icterus. Peripheral smear revealed atypical plasma cells (53%). Serum protein electrophoresis showed a monoclonal IgG-K paraprotein at a concentration of 4.56 (45 g/l) (Ref- 0.10-0.40). Patient had hypoalbuminemia and raised liver enzymes. The serology test for HIV was reactive for HIV-I and II. Bone marrow examination showed hypercellularity with near replacement of the normal marrow elements by sheets of mature plasma cells and a few immature plasma cells. Plasma cells were EBER positive. Based on the overall findings, a diagnosis of PPCL was made and subsequently, the patient was referred for chemotherapy.</p> <p><strong>Conclusion:</strong> To our knowledge PPCL in HIV infected young male associated with EBV has not been previously reported in the Indian literature. Given the rare incidence of this entity in the general population, it is very unlikely that they occurred by chance. Further research is needed to determine what would be the optimal management options of such patients.</p> Rakhi V. Jagdale, Jaydeep N. Pol, Zeba Nisar, Sachin J. Patil, Abhijit S. Pethkar, D. N. Lanjewar ##submission.copyrightStatement## Sat, 07 Mar 2020 00:00:00 +0000 A Clinically Asymptomatic Patient with a Flowcytometry Profile of Mycosis Fungoides/Sezary Syndrome <p><strong>Introduction: </strong>Mycosis fungoides (MF) is the most common type of cutaneous T –cell lymphoma accounting for 50% of all cutaneous lymphomas. Sezary Syndrome (SS) and MF are closely related T –cell neoplasms. They are considered separately on the basis of clinical features and cell of origin. Flowcytometry plays an important role in the diagnosis of MF/SS with a characteristic immunophenotypic expression of a lack of CD 7 as a common feature in all stages of the disease. Our case is a clinically asymptomatic patient with a flowcytometric pattern of Mycosis fungoides/Sezary syndrome. There is no documentation of such a case in the literature.</p> <p><strong>Case: </strong>A 55 - year – old male presented with persistent lymphocytosis. Investigations revealed a Hb of 15.5 g/dl, ESR 03 mm/1st hour, platelet count of 178,000/cu mm, total WBC count of 10,700/cu mm and an absolute lymphocyte count of 7000/cu mm (62%). The serum protein electrophoresis was normal. LDH was 149 IU/L (150-250) IU/L. A chest X-ray showed no pathology.</p> <p>The patient was followed up for a period of five months with full blood counts (FBC), monospot test, ultrasound scan of abdomen, full body CT scan, LDH level and viral studies. During this period, the lymphocytosis persisted. Serial absolute lymphocyte counts were 6332/cu mm, 4918/cu mm, 5749/cu mm, 6890/cu mm and 7820/cu mm. Viral studies revealed CMV IgG antibody positivity and Hepatitis A (HAV) IgG positivity. Studies for HIV were negative. Monospot test for infectious mononucleosis was negative. Ultrasound scan of abdomen and full body CT scan were normal. Blood picture revealed small to medium sized lymphocytes with scanty cytoplasm. Bone marrow examination revealed a reactive marrow with a normal lymphocyte count of 15-20%. Flowcytometry was performed using peripheral blood and bone marrow samples 5 months apart.</p> <p><strong>Results: </strong>Flowcytometry of peripheral blood and bone marrow revealed that T-lymphocyte<span style="text-decoration: line-through;">s </span>percentage was 92.0%and 82.0%, respectively. The immunophenotypic results for both blood and bone marrow revealed almost identical findings on LST (Lymphoid Screening Tube) and TCLPD (T Cell Lymphoproliferative Disorder) panels showing bright positivity of smCD3, CD4, CD2, TCRαβ, CD5 and dim positive CD8. Negative results were CD7, TCRγδ, CD25, CD26 and CD4+/ CD8+ ratio was 3.0/2.3 respectively.</p> <p><strong>Conclusion: </strong>The criteria of International Society of Cutaneous Lymphomas (ISCL) and the European Organization for Research and Treatment of Cancer (EORTC) to stage Mycosis fungoides and Sezary Syndrome were not present in our patient who was asymptomatic but showed a typical immunophenotypic pattern of MF/SS.</p> C. C. Kariyawasan, B. L. T. Balasuriya, S. A. C. D. Ranatunga ##submission.copyrightStatement## Fri, 27 Mar 2020 00:00:00 +0000 Effects of Hormonal Contraceptives on Haematological Parameters among Women in the Cape Coast Metropolis, Ghana <p><strong>Background:</strong> The widespread use of hormonal contraceptives gives grounds for assessing their influence on various biochemical parameters of the human system since its safety has become controversial. This study assessed the effects of hormonal contraceptives on haematological parameters among users in the Cape Coast Metropolis.</p> <p><strong>Materials and Methods:</strong> A simple randomized case-control study approach was used to recruit 94 healthy women of which 54 were hormonal contraceptive users and 40 healthy age-matched non-contraceptive users served as controls. Venous blood samples were taken for full blood count (FBC) analyses using an automated haematology analyser. Data was analysed using SPSS (V.22.0). Data was expressed in means (Mean ±SD) for the different variables. T-test statistic was used to compare the mean scores of two groups whilst one-way ANOVA was used to compare more than two groups. Pearson correlation was used to determine association between the various parameters. P-value &lt;0.05 was considered statistically significant.</p> <p><strong>Results:</strong> The study observed mean red blood cell (RBC) count to be significantly higher among hormonal contraceptive users compared to non-users (P=0.030). Additionally, the duration of contraceptive usage had an influence on the blood cell parameters in various ways, with a significant negative correlation between duration of contraceptive use and red cell distribution width (RDW) (r= -0.303, P= 0.026).</p> <p><strong>Conclusion and Recommendation:</strong> Hormonal contraceptives cause significant increase in red blood cell (RBC) count among users. Its effects also depend largely on duration of contraceptive use. The main limitation to this study was that blood films were not prepared to confirm the &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;results the haematology analyser, hence study is recommended with the incorporation of blood films.</p> Sampson Coffie, Albert Abaka-Yawson, Solomon Sosu Quarshie, Elliot Akorsu, Philip Apraku Tawiah, George Yiadom Osei, Patricia Mante, Joseph Adu-Amankwaah ##submission.copyrightStatement## Fri, 06 Mar 2020 00:00:00 +0000 Demographic and Clinical Enumeration of Patients of CLL: Experience of the Hematology-oncology Department of the Central Hospital of Yaounde <p>The objective of this study was to identify the demographic and clinical features of patients with chronic lymphocytic leukemia (CLL), followed at the Central Hospital of Yaoundé (HCY) over a period of 6 years (January 2012 to December 2018).</p> <p><strong>Methodology:</strong> This retrospective analytical study was carried out at the Central Hospital of Yaoundé at the Department of Hematology and Medical Oncology. We have selected from the archives the files of patients with chronic lymphocytic leukemia confirmed by blood immunophenotyping over a period of 6 years. Were includes the records of patients with documented CLL. The variables studied were: Epidemiological, clinical data and blood immunophenotyping. The data was analyzed using SPSS 20 software.</p> <p><strong>Results:</strong> Thirty-eight (38) patients were retained, all of them were of proliferation B. Their mean age was 58.5 years (52.50-68.25) with a female predominance (65.78%). The education level was that of primary school (41.66%). Marital status was dominated by the married couples (66.66%). Most of the patients were from the central region (58.62%). Eight patients (27.58%) were housewives. The most clinical presentation was splenomegaly (68.42%). White blood cells ranged from 48150 to 131075 / mm<sup>3</sup> with an average of 75850 / mm<sup>3</sup>; the average hemoglobin level was 9 g / dl (7.175 and 11.975 g / dl) and that of platelets was 134000 / mm<sup>3</sup> (81250 to 174,250 / mm<sup>3</sup>). According to the Matutes classification, 81.49% had a score of 5 and 18.51% of 4. Binet stage C disease was predominant in 60.52% of the cases.</p> <p><strong>Conclusion:</strong> Chronic lymphoid leukemia is a pathology that is still little-known; patients reach an advanced stage of the disease with splenomegaly and anemia. The limited technical platform and financial difficulty do not allow to have the optimal balance sheet in our context.</p> Chetcha Chemegni Bernard, F. Ngo Sack, A. Sango, E. Lontsi, N. Njedock ##submission.copyrightStatement## Tue, 31 Mar 2020 00:00:00 +0000