Genetic Polymorphisms of MTRRA66G Genes and Susceptibility to Acute Lymphoblastic Leukemia in the Yemeni Population
Published: 2022-12-05
Page: 171-178
Issue: 2022 - Volume 5 [Issue 4]
Radfan Saleh
Faculty of Medicine and Health Science, Taiz University, Yemen.
Hassan Hussein Musa
Faculty of Medical Laboratory Science, University of Khartoum, Sudan.
Gamal Abdul Hamid *
Faculty of Medicine, University of Aden, Yemen.
Muzamil M. Abdel Hamid
Faculty of Medical Laboratory Science, University of Khartoum, Sudan and Institute of Endemic Disease, University of Khartoum, Sudan.
*Author to whom correspondence should be addressed.
Abstract
Background: Folic acid metabolism has a major role in DNA methylation and synthesis. Genetic Polymorphisms in folate may disrupt enzyme activities and maybe affect the malignant risk.
Methionine synthase reductase <MTRR> are very important enzyme for the folate cycle.
Objectives: To study the possible relation between polymorphisms of MTRRA66G genes and susceptibility to acute lymphoblastic leukemia (ALL) in Yemeni patients.
Methods: A total of 115 patients with ALL attended oncology centers in Yemen and 140 unrelated healthy individuals as a control group was involved in a case-control study. DNA was extracted from collected EDTA venous blood samples and analyzed by polymerase chain reaction-restriction fragment length polymorphism assay [PCR-RFLP].
Results: The frequency of MTRR 66A-G heterozygous (AG), homozygous (GG) and wild type (AA) in cases was 52.2% (60), 22.6% (26), and 25.2% (29), respectively. Whereas, the frequency of MTRR 66A-G heterozygous (AG), homozygous (GG) and wild type (AA) in controls was 46.4% (65), 27.1% (38), and 26.4% (37), respectively. The difference in frequencies were statistically insignificant (P=0.471, OR = 1.065, 95% confidence interval (CI) 0.606–1.873).
Conclusion: Our finding for MTRR A66G polymorphism does not associate with the development of acute lymphoblastic leukemia in Yemeni patients.
Keywords: Acute lymphoblastic leukemia, methionine synthase reductase, polymorphism, Yemen