Validation Hematological Analyzer for Assay of Erythrogram in Hodeidah City, Yemen
Asian Hematology Research Journal,
The hematological indices namely erythrogram is an important parameter for evaluating human’s physiological status. I can vary accordingly depending upon the differential traits (Asian, Arabian, European … etc), sex, age, and health condition. In best of our knowledge, due to lack of researches in absence of constant values in this area this study is aimed to identify the reference values of erythrogram in the adults at Hodeidah, Yemen. The hematological analyzer (Sysmex KX-21) was validated for assessing the linearity, accuracy, precision and quantification with limit of different blood samples and was used for sampling in research analysis. Participated volunteers of this study were provided written consent, by following the university of Hodiedah and Office of Health and Public declaration. The erythrogram parameters include hemoglobin (Hb), red blood cells (RBC), packed cell volume (PCV), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC), for two investigated groups amongst whom 500 were males and 500 were females. Obtained data were analyzed. The results of validated method were precise to each analyze with percent relative standard deviations (RSD %) of intra-assay and inter – assay (< 5.0%) methods. Furthermore, the accuracy of validated method exhibit well recovery values of ± 5% and the coefficient correlation (R2) value was more than 0.9995 as a good linear method. On the other hand, the results showed the level of Hb, RBC, and PCV to be significantly different from males than that of females (p < 0.05). The erythrogram parametric means of this study were constant with the international normal range except for the MCH in both the groups, whereas reduction of Hb is found from the study. These variations might be related to the case of infectious diseases, but low in nutritional values and the geographical location of Hodeidah city, Yemen.
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