Asian Hematology Research Journal

The Hematological Profile, Hepatic Marker, and Histomorphology Effects on Canned Tomato Consumption Using Adult Male Wistar Rats

Ezon-Ebidor Innocent Edibamode *

Department of Anatomy, Faculty of Basic Medical Sciences, University of Port Harcourt, Nigeria.

Kanayo Mercy Odia *

Department of Human Physiology, Faculty of Basic Medical Sciences, University of Port Harcourt, Nigeria.

Nicholas Asiwe *

Department of Anatomy, Faculty of Basic Medical Sciences, University of Port Harcourt, Nigeria.

Edet Iboro Efiong *

Department of Human Anatomy, Faculty of Basic Medical Sciences, University of Uyo, Nigeria.

Favour Somtochukwu Ibeaha *

Department of Biomedical Technology, School of Science and Laboratory, University of Port Harcourt, Nigeria.

Mercy Omasirichi Ndumati *

Department of Biomedical Technology, School of Science and Laboratory, University of Port Harcourt, Nigeria.

*Author to whom correspondence should be addressed.

---

Abstract

Objective: The study investigates the effects of canned tomato consumption on the hematological profile, hepatic markers and histology.

Methods: The study involved 20 male Wistar rats divided into two groups, each fed Gino tomato sachets for 21 days. The rats were anesthetized, and liver and blood were extracted. Standard laboratory analysis, light microscope, GraphPad Prism, and ANOVA were used for descriptive and statistical analysis, with a significance level of p<0.05.

Results: Findings show the presence of Carbohydrates, Alkaloids, Flavonoids, reducing sugar and Resins were present, Results for PCV were 7.00±4.15 and 53.00±3.69%, Hb levels were 11.70±1.03 and 17.68±1.22g/L. (WBC) are 9.44±1.32 and 7.16±0.50g, with results significant at (p<0.05), in table 2. The results for Liver function are 12.80±2.78 and 4.80±0.58 U/l for AST, 8.60±1.33 and 5.40±0.68 U/L for ALT, Total protein 62.40±6.52 and 68.60±8.41 (g/L), and was significant at p<0.05 in table 4. The weights of the rats for the first 21 days were 81.70±3.20 and 84.50±3.46g, 102.00±3.45 and 114.90±3.84g, and 103.30±5.19 and 107.70±5.47g, with significance at week 2 in table 4, the weight after 21 days were 173.20±7.45 and 183.20±6.69g, 177.00±6.64 and 198.20±12.46g, and 189.60±8.01 and 205.40±9.90g.

Conclusion: Solanum lycopersicum had no significant effect on the hematological, histological, or hepatic markers of rats fed tomato pastes, implying no effect on their weight.

Keywords: Consumption, hematological parameters, anesthetizing, standard error of mean

---

---

---

References

Ali MY, Sina AA, Khandker SS, Neesa L, Tanvir EM, Kabir A, Khalil MI, Gan SH. Nutritional composition and bioactive compounds in tomatoes and their impact on human health and disease: A review. Foods. 2020 Dec 26;10(1):45.

Kumar A, Kumar V, Gull A, Nayik GA. Tomato (Solanum Lycopersicon). Antioxidants in vegetables and nuts-Properties and health benefits. 2020: 191-207.

Collins EJ, Bowyer C, Tsouza A, Chopra M. Tomatoes: An extensive review of the associated health impacts of tomatoes and factors that can affect their cultivation. Biology. 2022 Feb 4;11(2):239.

Jaglan P, Buttar HS, Al-awarded OA, Chibisov S. Potential health benefits of selected fruits: Apples, blueberries, grapes, guavas, mangos, pomegranates, and tomatoes. In Functional Foods and Nutraceuticals in Metabolic and Non-Communicable Diseases 2022 Jan 1; 359-370.

Berding K, Vlckova K, Marx W, Schellekens H, Stanton C, Clarke G, Jacka F, Dinan TG, Cryan JF. Diet and the microbiota–gut–brain axis: Sowing the seeds of good mental health. Advances in Nutrition. 2021 Jul 1;12(4):1239-85.

Rastogi R, Chaturvedi DK, Satya S, Arora N. Intelligent heart disease prediction on physical and mental parameters: An ML based IoT and big data application and analysis. Machine learning with health care perspective: Machine learning and healthcare. 2020:199-236.

Parmar M, Rajput H, Johri S. Solanum lycopersicum, a Reservoir of Antioxidants. In Antioxidant-based therapies for disease prevention and management. 2021 Dec 22;253-268.

García-Alonso FJ, García-Valverde V, Navarro-González I, Martín-Pozuelo G, González-Barrio R, Periago MJ. Tomato. In Nutritional Composition and Antioxidant Properties of Fruits and Vegetables. 2020 Jan 1;255-271.

Wu X, Yu L, Pehrsson PR. Are processed tomato products as nutritious as fresh tomatoes? Scoping review on the effects of industrial processing on nutrients and bioactive compounds in tomatoes. Advances in Nutrition. 2022 Jan 1;13(1): 138-51.

Wang C, Li M, Duan X, Abu-Izneid T, Rauf A, Khan Z, Mitra S, Emran TB, Aljohani AS, Alhumaydhi FA, Thiruvengadam M. Phytochemical and nutritional profiling of tomatoes; impact of processing on bioavailability-A comprehensive review. Food Reviews International. 2023 Sep 8;39(8):5986-6010.

Mieszczakowska-Frąc M, Celejewska K, Płocharski W. Impact of innovative technologies on the content of vitamin C and its bioavailability from processed fruit and vegetable products. Antioxidants. 2021 Jan 5;10(1):54.

Sadler CR, Grassby T, Hart K, Raats M, Sokolović M, Timotijevic L. Processed food classification: Conceptualisation and challenges. Trends in Food Science & Technology. 2021 Jun 1;112: 149-62.

Sá AG, Moreno YM, Carciofi BA. Food processing for the improvement of plant proteins digestibility. Critical Reviews in Food Science and Nutrition. 2020 Nov 12;60(20):3367-86.

Cheema HS, Singh MP. The use of medicinal plants in digestive system related disorders—A systematic review. J. Ayurvedic Herb. Med. 2021; 7(3): 182-7.

Shi J, Maguer ML. Lycopene in tomatoes: Chemical and physical properties affected by food processing. Critical Reviews in Food Science and Nutrition. 2000 Jan 1;40(1):1-42.

Arballo J, Amengual J, Erdman Jr JW. Lycopene: A critical review of digestion, absorption, metabolism, and excretion. Antioxidants. 2021 Feb 25;10(3):342.

D’Introno A, Paradiso A, Scoditti E, D’Amico L, De Paolis A, Carluccio MA, Nicoletti I, DeGara L, Santino A, Giovinazzo G. Antioxidant and anti‐inflammatory properties of tomato fruits synthesizing different amounts of stilbenes. Plant Biotechnology Journal. 2009 Jun;7(5):422-9.

Dominic S, Hussain AI, Saleem MH, Alshaya H, Jan BL, Ali S, Wang X. Variation in the primary and secondary metabolites, antioxidant and antibacterial potentials of tomatoes, grown in soil blended with different concentration of fly ash. Plants. 2022 Feb 19;11(4): 551.

Buabeid M, Gacem SA, Fujihashi A, Trish A, Nadar RM, Govindarajulu M, Dhanasekaran M. Neuroprotective Effects of Portulaca oleracea and Portulaca quadrifida Linn. Medicinal Herbs and Fungi: Neurotoxicity vs. Neuroprotection. 2021:495-510.

Amaechi D, Ekpe IP, Edet ED, Madu MC. Hepatoprotective and hematological effects of Solanum melongena (garden egg), Solannum lycopersicum (tomato) and daucus carrots Subsp. sativus (carrot) extracts against lead induced toxicity in Wistar rats. Asian Journal of Biochemistry, Genetics and Molecular Biology. 2021; 7(2):10-8.

Buabeid MA, Arafa ES, Rani T, Ahmad FU, Ahmed H, Hassan W, Murtaza G. Effects of Solanum lycopersicum L.(tomato) against isoniazid and rifampicin induced hepatotoxicity in wistar albino rats. Brazilian Journal of Biology. 2022 Feb 7;84:e254552.

Roche M, Rondeau P, Singh NR, Tarnus E, Bourdon E. The antioxidant properties of serum albumin. FEBS letters. 2008 Jun 11;582(13):1783-7.