Establishment of Haematological Reference Values in Healthy Undergraduates (Aged 16-35 Years) in Part of South-South Nigeria
Babatope, I.O. *
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Momodu Nasiru, F.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Mordi, O.J.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Keshinro, O.O.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Aisowieren, F.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Airende, Q.L.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Osezua, R.O.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Omijie, F.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Enwefa, E.M.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Osayande, W.O.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Olabhie, F.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Okodugha, C.O.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
Buoro, P.
Department of Haematology and Blood Transfusion Science, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Interpretation of laboratory test results with appropriate diagnostic accuracy requires reference or cutoff values.
Aims: This study is aimed at establishing reference values of some haematological parameters among healthy undergraduate students (aged 16-35 years) in South-South Nigeria.
Methodology: A total of One thousand nine hundred and thirty-five healthy students of Ambrose Alli University, Ekpoma were recruited into this study. Only subjects negative for HIV I & II, HBsAg, HCV, VDRL and belonged to Haemoglobin genotype AA, AS or AC were included in this study. Haematological parameters of the subjects were analyzed using Sysmex autoanalyzer.
Results: Our results showed the reference intervals of 3.6835-5.82, 9.9-15.2, 29.8-44.5, 80.36-86.7, 22.18-30.2, 30.6-36.2, 33.9-45.9 and 11.8-16.1 were obtained for the respective parameters of RBC, PCV, MCV, MCH, MCHC, RDW SD and RDW CV. WBC parameters such as WBC, LYM %, MXD %, NEUT %, LYM (x103/µl), MXD (x103/µl) and NEUT (x103/µl) recorded reference intervals of 3.1-7.8, 23.7625-62.8, 4.7-20.0, 37.78-60.9, 1.3-3.7, 0.2-1.135 and 0.9-4.0 respectively. Furthermore, the Platelets parameters of PLT, PDW, MPV and P-LCR were 80-339.3, 10.2-18.3, 8.9-12.1 and 16.2275-41.7 respectively. With respect to sex, the male subjects had higher RBC, HGB PCV and MXD % than the female subjects. Whereas women had higher RDW SD, WBC, LYM (x103/µl), MXD (x103/µl), NEUT (x103/µl) and PLT than men. On the other hand, MCV, MCH, MCHC, RDW CV, LYM %, NEUT %, PDW, MPV and P-LCR were not affected by sex (P>0.05). Based on age, only the PCV and MCV of the study subjects were affected (P>0.05).
Conclusion: In conclusion, the haematologic reference values of the subjects studied were lower compared to reference ranges elsewhere. Furthermore, some haematological parameters were both affected by sex and age in the study area.
Keywords: Reference values, haematological parameters, undergraduates, laboratory, South-South