Haematological and Gross Pathological Changes in Broilers Experimentally Challenged with Velogenic Strain of Newcastle Disease Virus

Main Article Content

C. O. Faeji
M. K. Oladunmoye
I. A. Adebayo
T. T. Adebolu

Abstract

Newcastle disease (ND) is a severe and fatal disease of poultry caused by Newcastle Disease Virus (NDV). The disease is of economic and public health importance and has been a threat to the growth of poultry industry. A hundred and twenty day-old broiler chickens were procured from a commercial breeder farm. Glucose, vitamin, antibiotics were administered accordingly. Birds were tested for antibodies to NDV and vaccines were administered accordingly. Feed and water were also provided ad-libitum. Birds were randomly distributed into groups. Experimental birds were challenged at five weeks of age. Blood samples were collected for haematology assay periodically after challenging with NDV. The erythrocyte response in the chickens had varying patterns; there were significant reduction in values of Total Erythrocyte Count, Packed Cell Volume, Hemoglobin count in infected chickens. Monocytes were reduced significantly in the infected birds to 7±0.6% as compared to uninfected birds which had 10±0.9%. There were no significant changes in the Eosinophil and basophil absolute values in both infected and uninfected birds during the course of the study. This study showed that haematological values of broiler chicken significantly differ (p=< 0.05) from uninfected and infected birds with virulent NDV. Generally, there were no significant differences in the profile of the vaccinated birds. Hence the need for vaccination and research towards anti-NDV therapeutic discoveries cannot be overemphasized.

Keywords:
Heamatology, velogenic, Newcastle disease virus, broiler

Article Details

How to Cite
Faeji, C. O., Oladunmoye, M. K., Adebayo, I. A., & Adebolu, T. T. (2019). Haematological and Gross Pathological Changes in Broilers Experimentally Challenged with Velogenic Strain of Newcastle Disease Virus. Asian Hematology Research Journal, 2(1), 1-5. Retrieved from https://journalahrj.com/index.php/AHRJ/article/view/30102
Section
Original Research Article

References

Akele YR, Tatfeng YM, Ojiezeh IT, Chollom CS, Enitan SS, Olayanju OA. Evaluation of the efficacy of Newcastle disease (Lasota) live vaccines sold in Jos, Plateau State, Nigeria. European Science Journal. 2014;10(27):1857-7431.

Zhang Y, Zhang S, Wang X, Zhang G. Complete genome sequence of a sub-genotype vii d Newcastle disease virus circulating predominantly in chickens in Ch. Jour. of Vir. 2012;86(24):13849- 13850.

Alexander D. Newcastle disease and other avian paramyxoviruses. Rev. Sci. Tech. 2000;19(4):443-462.

Office International Des Epizooties (OIE). Manual of diagnostic tests and vaccines for terrestrial animals: Mammals, birds and bees. Biological Standards Commission. W. Org. for Anim. H., Paris. 2012;1-19.

Talebi S, Asri R, Rozeh C, Sahraei G. Comparative studies on haematological values of broiler strains (Ross, Cobb, Arbor-acres and Arian). Int. J. Poult. Sci. 2005;4(8):573-579.

Esonu B, Iheukwumere F, Emenalom O, Uchegbu M, Etuk E. Performance, nutrient utilization and organ characteristics of broilers fed Microdesmis puberula leaf meal. Jour. of Liv. Prod. Rural Dev. 2002;14(2):6-12.

Eze C, Okoye J, Ogbonna I, Ezema W, Eze D, Okwori E, Okorie-Kanu C, Idika I. Comparative evaluation of the effects of velogenic Newcastle disease virus infection on the hematology of ducks and chickens. Op. Jour. of Vet. Med. 2014;4(2):113-121.

Adeyemo I, Sani A. Haematological parameters and serum biochemical indices of broiler chickens fed Aspergillus niger hydrolyzed cassava peel meal based diet. IJRRAS. 2013;15(3):124-130.

Calderon N, Galindo F, Ortiz M, Lomniezi B, Fehervari T, Paaseh L. Thrombocytopenia in Newcastle disease: Haematological evaluation and histological study of bone marrow. Act. Vet. Hung. 2005;53(9):507-513.

Chekwube P, Osita A, Innocent O, Wilfred S, Didacus C, Emmanuel H, Christan K, Kalu I. Comparative evaluation of the effects of velogenic Newcastle disease virus infection on the hematology of ducks and chicken. Open J. Vet. Med. 2014;4(6):113-121.

Grogan K, Fernandez R, Barranon F, Espinosa H. Avian immune system: A brief review. Gaines. Jour. of Med. Sci. 2008;2(1):1-12.

Ojiezeh T, Ophori E. Haemogram and serum enzymes activities of Newcastle disease virus challenged broiler chickens following supplemental treatment with Aloe vera extract. J. Clin. Cell Immunol. 2015;6(2):282-296.

Graczyk S, Pliszczak-Krol A, Kotonski B, Willzek J, Chmielak Z. Examination of haematological and metabolic changes mechanisms of acute stress in Turkeys. Elect. Jour. of Pol. Agri. and Univ. Vet. Med. 2003;6(2):1-10.

Uchegbu M, Ezuma C, Ogbuewu I, Opara M. Physiological responses of finisher broilers to yam Peel meal: Haematology and serum biochemistry. Elect. Jour. of Env. Agr. and F. Chem. 2010;9(3):1657-1664.

Malik M, Sohail N, Sajid H, Hamidullah S, Bano N, Shah S. Effects of Newcastle disease virus on different haematological parameters in broilers. Adv. Anim. Vet. Sci. 2018;6(4):183-186.

Spinu M, Spinu O, Degen AA. Haematological and immunological variables in a domesticated and wild subspecies of ostrich (Struthio camelus). Br Poult Sci. 1999;40:613–8.

Balachandran P, Srinivasan P, Sivaseelan S, Balasubramaniam GA, Gopala Krishna Murthy TR. Isolation and characterization of Newcastle disease virus from vaccinated commercial layer chicken. Vet. World. 2014;7(7):457-462.

Panus A, Setiyaningsih S, Mayasari NLP. Newcastle disease virus infection study on duck and chicken in Subang District. J. Ilmu Ternak Vet. 2015;20(2):134-147.

Xiao S, Paldurai A, Nayak B, Samuel A, Bharoto AE, Prajitno TY, Collins PL, Samal SK. Complete genome sequences of Newcasle disease virus strains circulating in chicken populations of Indonesia. J. Virol. 2012;86(10):5969-5970.