Qualitative Phytochemical Screening, Anti-inflammatory and Haematological Effects of Alkaloid Extract of Combretum dolichopetalum Leaves

Main Article Content

Nwuke P. Chinedu


Background: Inflammation has been implicated in a number of wounds and disease conditions, spurring the search for compounds with anti-inflammatory properties.

Aim: The study investigated the anti-inflammatory potentials and effect of alkaloid extract of Combretum dolichopetalum on haematological parameters of Wistar albino rats.

Methodology: Alkaloids in the leaves were extracted using 10% acetic acid as solvent. The anti-inflammatory potential was investigated using egg-albumin paw edema model, while the haematological effects of the extract was observed on plasma.

Results: Phytochemical screening showed the presence of alkaloids, saponins and tannins in moderate concentration. Alkaloid specific test showed the presence of six classes of alkaloids. Result for anti-inflammatory potentials showed a significant (p<0.05) reduction in paw size of extract treated group compared to negative control. The extract also stabilized haematological parameters compared to negative control.

Conclusion: In conclusion, the study confirms the use of the alkaloid extract of Combretum dolichopetalum leaves in pain management and inflammatory conditions.

Alkaloids, Combretum dolichopetalum, phytocompounds, inflammation

Article Details

How to Cite
Chinedu, N. P. (2020). Qualitative Phytochemical Screening, Anti-inflammatory and Haematological Effects of Alkaloid Extract of Combretum dolichopetalum Leaves. Asian Hematology Research Journal, 3(3), 5-12. Retrieved from https://journalahrj.com/index.php/AHRJ/article/view/30132
Original Research Article


Asuzu IU, Adimorah RI. The anti-inflammatory activity of extracts from the root of Combretum dolichopetalum. Phytomedicine. 1998;5(1):25-28.

Gugu TH, Eze CO, Kenechukwu FC, Nnadi CO, Ofokansi KC, Attama AA, Esimone CO. In vitro and in vivo biological evaluations of Combretum dolichopetalum leave extract and fractions in the management of some tropical diseases. IOSR Journal of Pharmaceutical and Biological Science. 2019;14(1):47-60.

Asuzu IU, Onu OU. Anti-ulcer activity of the ethanolic extract of Combretum dolichopetalum root. Journal of Pharmaceutical Biology. 1990;28:27-32.

Samuel OO, Chinemerem E, Emmanuel E, Kelechi GM, Maxwell IE, Yusuf NO, Aruh OA. Antioxidant, anti-inflammatory and anti-nociceptive properties of hydro-methanol extract of Eremomastax speciosa (Hochst.) Cufod leaf. African Journal of Traditional Complementary and Alternative Medicine. 2017;14(6): 56-63.

Harborne JB. Phytochemical methods, Chapman and Hall, Ltd., London. 1973;49-188.

Harborne JB. Phytochemical methods, 2nd edition, Chapman and Hall, Ltd., London, New York. 1984;288.

Sofowora EA. Medicinal plants and traditional medicine in Africa, 3rd ed. Spectrum books Ltd, Ibadan, Nigeria. 2000;1.

Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rats as an assay for anti-inflammatory drugs. Exp. Biol. Med. 1962;111(3):544-547.

Ahmed AA, Mahmoud AA, Williams HJ, Scott AI, Reibenspies JH, Mabry TJ. New sesquiterpene a - methylene lactones from the Egyptian plant Jasonia candicans. J. Nat. Prod. 1993;56:1276-1280.

Perez GRM. Anti-inflammatory activity of Ambrosia artemisiaefolia and Rheo spathaceae. Phytomedicine. 1996;3:163-164.

Baker FJ, Silverton RE. Haemostasis and blood coagulation. In: FJ Baker, RE Silverton and CJ Pallister (Eds). Introduction to Medical Laboratory Technology. Sixth edition. Butterworth and Co. Sydney. 1985;312-330.

Hussein RA, El-Anssary AA. Plants secondary metabolites: The key drivers of the pharmacological actions of medicinal plants. Herbal Med. 2018;76139.

Yasmeen M, Prabhu B, Agashikar NV. Antidiarrheal activity of flowers of Ixora coccinea Linn. in rats. J. Ayurveda. Integr. Med. 2010;1(4):287-291.

Karou D, Dicko MH, Simpore J, Traore AS. Antioxidant and antibacterial activities of polyphenols from ethnoledicianla plants of Burkina Faso. Afr. J. Biotechnol. 2005;4:823-828.

Bribi N, Algeri F, Nogales AR, Garrido-Mesa J, Vezza T, Maiza FB, Utrilla PM, Rodriguez-Cabezas EM, Galvez J. Antinociceptive and anti-inflammatory effects of total alkaloid extract from Fumaria capreolata. Evi. Based Compl. Alt. Med. 2015;736895.

Vieira A. A comparison of traditional anti-inflammation and anti-infection medicinal plants with current evidence from biomedical research: results from a regional study. Pharmacog. Res. 2010;2(5):293-295.

Alam A, Subhan N, Alam S, Sarder M, Nahar L, Sarker S. Antinociceptive and anti-inflammatory properties if Ruellia tuberosa. Pharmaceutical Biol. 2010;47(3):209-214.

Balakumar C, Lamba P, Kishore DP, Narayana BL, Rao KV, Rajwinder K, Rao AR, Shireesha B, Narsaiah B. Synthesis, anti-inflammatory evaluation and docking studies of some new fluorinated fused quinazolines. European Journal of Medicinal Chemistry. 2010;45(11):4904- 4913.

Silva RO, Sousa FB, Damasceno SR, Carvalho NS, Silva VG, Oliveira FR, Sousa DP, Aragão KS, Barbosa AL, Freitas RM, Medeiros JVR. Phytol, a diterpene alcohol, inhibits the inflammatory response by reducing cytokine production and oxidative stress. Fundam. Clin. Pharmacol. 2014:(4):455–464.

Adeyemo OK. Haematological profile of Clarias gariepinus (Burchell, 1822) exposed to lead. Turk. J. Fish Aquat. Sci. 2007;163-169.

Kantarci A, Oyaizu K, Van Dyke T. Neutrophils-mediated tissue injury in periodontal disease pathogenesis: findings from localized aggressive periodontitis. J. Periodontitis. 2003;74(1):66- 75.