Some Haematological Parameters, Malondialdehyde and Glutathione Peroxidase Status of Diabetic Patients in Sokoto, Nigeria
Published: 2019-02-25
Page: 140-152
Issue: 2018 - Volume 1 [Issue 4]
Onuigwe Festus Uchechukwu *
Department of Haematology, School of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria
Abubakar Auwal Nasir
Department of Haematology, School of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
Erhabor Osaro
Department of Haematology, School of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
Buhari Hauwa Ali
Department of Haematology, School of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
Marafa Hamidu Ahmed
Department of Haematology, School of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
Bagudo Aliyu Ibrahim
Department of Haematology, School of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
Uchechukwu Nkechi Judith
Department of Chemical Pathology, Maryam Abacha Women and Children Hospital, Sokoto, Nigeria.
Amilo Grace Ifechukwudebelu
Department of Haematology, Faculty of Medicine, College of Health Science, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.
Ibeh Nancy Chitogu
Department of Medical Laboratory Sciences, Faculty of Health Science and Technology, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Background: World Health Organization statistics identify 150 million people with diabetes mellitus worldwide and suggest that this figure may double by 2025.
Aim: This research was conducted to determine the status of some haematological parameters and free radicals among diabetic patients attending Specialist Hospital, Sokoto.
Study Design: This was a case control study involving two groups; 29 controls (non-diabetic) and 58 diabetic subjects.
Methodology: The participants were divided into two groups; 29 controls (non-diabetic) and 58 diabetic subjects. Five milliliters (5 ml) of blood was collected into ethylenediaminetetraacetic acid (EDTA) and plain containers for haematological and free radicals’ analysis respectively. The full blood count (FBC) investigation was carried out using automated haematology analyser while malondialdehyde (MDA) and glutathione peroxidase status (GPx) investigations were carried out using chemical and enzymatic methods respectively. The FBC, GPx and MDA status of both control and subjects were compared in this study.
Duration: The study lasted for a period of six months between April to September, 2017.
Results: The results obtained in this study showed a significant increase in WBC, neutrophil, eosinophil and Platelet counts of diabetic subjects (P ≤ 0.05) when compared with controls, while other FBC parameters, MDA and GPx were not significant (P> 0.05). There was significant decrease in the basophil count of the diabetic subjects based on marital status (P ≤0.04), while other parameters measured were not significant (P> 0.05). The result also showed a significant increase in the MDA of diabetic subjects based on age (P≤ 0.05), while others were not significant (p>0.05). the study also showed no statistical difference in the FBC, MDA and GPx of the smokers and non-smokers (P˃0.05)
Conclusion: This research shows that WBC count, neutrophil, eosinophil and platelet count of the diabetic patients increased. There is need for a further research on direct free radical estimation and total antioxidant status in relation to diabetes, which gives more information than the indirect method of estimation. It is recommended that white blood cells and platelet levels should be closely monitored for proper management diabetic patients.
Keywords: Haematological, parameters, free radicals, diabetic, patients, Sokoto