Asian Hematology Research Journal https://journalahrj.com/index.php/AHRJ <p style="text-align: justify;"><strong>Asian Hematology Research Journal</strong>&nbsp;aims to publish&nbsp;high-quality&nbsp;papers (<a href="/index.php/AHRJ/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of&nbsp;‘Hematology research’. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated.By not excluding papers based on novelty, this journal facilitates the research and wishes to publish papers as long as they are technically correct and scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled, OPEN peer-reviewed, open-access INTERNATIONAL journal.</p> <p>&nbsp;</p> Asian Hematology Research Journal en-US Asian Hematology Research Journal Observational Study on Dual Marker of T Cell Activation for Early Diagnosis of Hemophagocytic lymphohistiocytosis (HLH) on Peripheral Blood https://journalahrj.com/index.php/AHRJ/article/view/30212 <p><strong>Introduction:</strong> Hemophagocytic lymphohistiocytosis (HLH) is a fatal disorder, characterized by a cytokine storm due to cytotoxic T cell activation, the causes of which are various. Its detection is first on clinical awareness and proved by several tests. However, there is not one specific test to diagnose HLH. We present an observational study to assess T cell activation dual marker (CD 38 high/ HLA -DR) as one of the important diagnostic parameters for HLH for an early rapid diagnosis for this condition.</p> <p><strong>Methodology:</strong> This is a retrospective observational study conducted in 22 patients. Demographic information, clinical characteristics, diagnostic impressions, pathological features, and laboratory values were collected for each patient. Flow cytometric immunophenotyping for dual positive markers was performed. The panel included antibodies specific for CD45, HLA-DR, CD3, CD4, CD8 and CD 38 T-cell receptor (TCR).</p> <p><strong>Results:</strong> Routine markers such as soluble CD 25, CRP, LDH, triglycerides, LDH and ferritin were unable to differentiate between HLH and other similar conditions. T-cell activation panel performed in these patients showed that 63.64% patients were positive and 36.36% negative for dual markers ((CD 38 high/ HLA -DR)).</p> <p><strong>Conclusion:</strong> T cell activation marker CD 38 high/ HLA -DR was found to be a rapid marker for identification of HLH. This will aide clinicians with a rapid specific diagnosis although the benefit of an early diagnosis did not yield a better outcome indicating that a larger study is needed.</p> Abhay A. Bhave Sarang Waghmare Nitin Chavan Kashvi Mehta Fatema Rangwalla Shrinath Kshirsagar ##submission.copyrightStatement## 2023-01-05 2023-01-05 1 7 Clinical Significance and Outcome of COVID-19 Patients with Thrombocytopenia in Intensive Care Unit: A Single Centre Study https://journalahrj.com/index.php/AHRJ/article/view/30213 <p><strong>Introduction: </strong>SARS-CoV-2 infection can cause manifestations in multiple systems in the body. Haematological system can also be affected by both SARS-CoV-2 infection and critical illness. There have been studies that show thrombocytopaenia is connected with SARS-CoV-2 infection.&nbsp; The data on its prevalence and outcome on critically ill COVID-19 patients are scarce. Some studies have shown that thrombocytopaenia is associated with worsening of respiratory &nbsp;&nbsp;parameters and unfavourable outcome of the critically ill patients. Identifying the presence of thrombocytopaenia and its consequences is important to manage critically ill COVID-19 patients.</p> <p><strong>Aims: </strong>To identify the prevalence and consequences of thrombocytopaenia, the presence of other causes of thrombocytopaenia and association of thrombocytopenia with the outcome in critically ill COVID-19 patients.</p> <p><strong>Study Design: </strong>A retrospective clinical analysis.</p> <p><strong>Study Set Up: </strong>This was done in COVID designated Intensive care unit and High dependency unit in Base Hospital- Teldeniya, Sri Lanka.</p> <p><strong>Methodology: </strong>A retrospective study was done at COVID designated intensive care unit- Base Hospital, Teldeniya, Sri Lanka. All patients with positive SARS-CoV-2 testing who later develops pneumonia with oxygen dependency and requiring ICU or HDU care were included in the study. Data was collected from patient records for the duration from 1<sup>st</sup> of January 2021 to 30<sup>th</sup> of June 2021. Their demographic data, data related to platelet counts, complications, outcome and other causes leading to thrombocytopaenia were collected. Thrombocytopaenia was categorised as mild (platelet count 100-150×10<sup>9</sup>/L), Moderate (50-100×10<sup>9</sup>/L) and severe (less than 50×10<sup>9</sup>/L).</p> <p><strong>Results: </strong>Total of 189 patients were admitted to either ICU or HDU requiring oxygen therapy due to COVID pneumonia during the study period. The mean age was 60.59 years with SD of 14.9. Age range was 16 to 94 years.&nbsp; 63 patients (33.33%) had thrombocytopenia with 8 (12.69%), 19 (30.15%) and 36 (57.14%) had severe, moderate and mild thrombocytopenia respectively. 28 (44.4%) of the patients had low platelets at the time of admission. 31.7% of the patients had another cause that could contribute for thrombocytopenia. In thombocytapanic patients, there were significantly elevated levels of C-reactive protein and serum creatinine. Twenty (31.7%) of the patients with thrombocytopenia died. This accounted for 48.7% of all critical care deaths. When compared to patients with a normal platelet count, the proportion of deaths in patients with thrombocytopenia was found to be significant. (p=0.045).</p> <p><strong>Conclusions: </strong>In critically ill patients, thrombocytopenia can occur due to a variety of causes. According to our findings, at least one-third of critically ill patients with COVID-19 infection develop thrombocytopenia at some point during their illness. This group has a high mortality rate. It is critical to understand the progression of the illness as well as the outcome in order to plan discharge and follow-up for these patients.</p> Ashani Ratnayake Ayeshani Rajapakse Prabhashini Kumarihamy ##submission.copyrightStatement## 2023-01-09 2023-01-09 8 15 Assessment of Renal Functions among Sudanese Patients with Acute Lymphoblastic Leukemia's Receiving Chemotherapy in Radiation and Isotopes Center of Khartoum (RICK), Sudan https://journalahrj.com/index.php/AHRJ/article/view/30214 <p><strong>Background:</strong> A wide array of disturbances in electrolyte equilibrium is commonly seen in patients with acute leukemia (AL). These abnormalities present a potential hazard in these patients, as that of enhancing the cardio-toxic effects of certain chemotherapeutic regimens.</p> <p><strong>Materials and Methods:</strong> This study was designed as interventional study, which includes 50 samples, the data was collected form hospital archives, include both male and female (the males is 24 with 48% while the rests 26 is females with 52%), from different area in Sudan (east is 2%, west is 64%, north is 10%, south is 12%, and center is 12%), have different classification of Acute Lymphoblastic Leukemia's (L1 is 17 with 34%, L2 is 27 with 54% and L3 is 6 with 12%). Carried out in Radiation and Isotopes Center of Khartoum in Khartoum state, during the period from February 2020 to January 2021, and the obtained data were analyzed by SPSS.</p> <p><strong>Results:</strong> The result of this study showed that there was insignificant difference (p 0.05) in the serum levels of urea, creatinine, uric acid, sodium, calcium, phosphorous, and magnesium in the study groups before and after treatment, and significant increase in the serum level of potassium in the study groups after treatment p. value of 0.007, the mean of serum level of potassium before and after treatment is 2.688 and 3.702 respectively. And insignificant difference (p 0.05) in the serum levels of urea, creatinine, uric acid, sodium, calcium, phosphorous, potassium and magnesium in the study groups before and after treatment according to gender and FAB classification.</p> <p><strong>Conclusion:</strong> The study conclude that the serum levels of urea, creatinine, uric acid, sodium, calcium, phosphorous, and magnesium was not affected by treatment, but the potassium is increased after treatment. And the gender and classification of Acute Lymphoblastic Leukemia's have insignificant effect on the serum levels of urea, creatinine, uric acid, sodium, calcium, phosphorous, potassium and magnesium.</p> Salman Taha Ahmed Elmukashfi Walaa Maryoud Aljack Mihad Magboul Ahmed Abdelrahman Mohamed Sidahmed Mohamed Ahmed Yasin Hassan ##submission.copyrightStatement## 2023-01-10 2023-01-10 16 22 Assessment of Essential Trace Elements and Toxic Metal Contaminants of Banked Blood Designated for Transfusion in Igbinedion University Teaching Hospital, Okada, Nigeria https://journalahrj.com/index.php/AHRJ/article/view/30215 <p>Blood transfusion is a critical part of patient’s intensive care and is life saving for patients with severe anemia or hemorrhage. The goal of blood banking is to provide adequate and safe blood to recipients at no risk to donors. These donors may have had a variety of exposures to substances including toxic heavy metals from environmental and occupational sources. Exposure to environmental heavy metals is common among Africans. Although many of these metals are known neurotoxicants, to date, monitoring of this exposure is limited, even in countries such as Nigeria that are undergoing rapid industrialization. Concentration of Lead, Cadmium, Zinc and copper in samples from eighty six (86) blood donors comprising of O, A, B and AB blood groups were estimated by inductively Coupled Plasma Mass Spectrometer (ICP-MS), aliquot of 20µL of blood sample was aspirated into the quartz spray chamber after instrument was standardized with standard blank and various standards (Cadmium, Lead, Zinc and Copper). Data values of Lead, Cadmium, Zinc and Copper obtained indicated that essential and toxic metal levels from donor blood were within permissible range. Data were analysed using SPSS version 20 and significance level were set at p =≤ 0.05. The observed blood levels of cadmium and lead in donor blood banked designated for transfusion in this study were in correlation with the permissible range of toxic metals. More so, there was an insignificant increase in the essential metals level in the blood donor group when compared with the reference range. This study therefore concludes that donor blood designated for transfusion at Igbinedion university teaching hospital Okada had metal concentrations that is within the estimated tolerable concentration.</p> Mitsan Olley Sule Zekeri Josiah Joel Sunday Aiyanyor David Osayomwanbo Awa Favour Chinatu ##submission.copyrightStatement## 2023-01-19 2023-01-19 23 30