Asian Hematology Research Journal https://journalahrj.com/index.php/AHRJ <p style="text-align: justify;"><strong>Asian Hematology Research Journal</strong>&nbsp;aims to publish&nbsp;high-quality&nbsp;papers (<a href="/index.php/AHRJ/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of&nbsp;‘Hematology research’. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated.By not excluding papers based on novelty, this journal facilitates the research and wishes to publish papers as long as they are technically correct and scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled, OPEN peer-reviewed, open-access INTERNATIONAL journal.</p> <p>&nbsp;</p> en-US contact@journalahrj.com (Asian Hematology Research Journal) contact@journalahrj.com (Asian Hematology Research Journal) Wed, 31 Aug 2022 11:18:02 +0000 OJS 3.1.1.4 http://blogs.law.harvard.edu/tech/rss 60 Utility of Immunophenotyping at Diagnosis in Multiple Myeloma-an Observational Study from a Tertiary Care Center in India https://journalahrj.com/index.php/AHRJ/article/view/30203 <p><strong>Introduction and Aims: </strong>Flow cytometry (FCM) has been useful in differentiating abnormal plasma cells (APCs) from normal plasma cells (NPCs) based on the different surface antigen expressions as well as the clonality analysis. The enumeration of NPCs and APCs is of prognostic significance. The phenotypic expressions of different antigens have been found prognostically relevant in myeloma. We intended to evaluate the prognostic significance and utility of immunophenotyping of multiple myeloma (MM) cases at diagnosis.</p> <p><strong>Materials and Methods:</strong> We evaluated 48 newly diagnosed MM cases. Immunophenotyping was done by a 3-tube 6-color FCM panel. NPCs and APCs were defined based on light chain restriction and immunophenotypic aberrancies.</p> <p><strong>Results:</strong> Cases with &gt;3% NPCs did not show a RISS3 disease (P=0.024). Cases with &lt;3% NPCs at diagnosis were predominantly ISS2 or ISS 3 disease. Cases with &gt;3% NPCs in bone marrow presented with a higher hemoglobin (P=0.004), lower creatinine (P=0.035) and lower beta-2 microglobulin (P=0.003) compared to cases with &lt;3% NPCs at diagnosis.</p> <p><strong>Conclusion:</strong> Cases with increased number of NPCs at diagnosis are associated with good risk factors and may fare well after treatment. A diagnostic immunophenotyping should be encouraged to find out sub-groups of MM patients with higher NPCs.</p> Asish Rath, Tribikram Panda, Jasmita Dass, Tulika Seth, Manoranjan Mahapatra, Seema Tyagi ##submission.copyrightStatement## https://journalahrj.com/index.php/AHRJ/article/view/30203 Wed, 31 Aug 2022 00:00:00 +0000 Inhalation Effect of Insecticides on Coagulation Profiles of Rabbits https://journalahrj.com/index.php/AHRJ/article/view/30204 <p><strong>Background:</strong> High malaria burden&nbsp;&nbsp; has led to the increase use of insecticides in the tropics and subtropics. This study thus aimed at assessing the effect of insecticides inhalation on coagulation profile using experimental animal model.</p> <p><strong>Methods:</strong> Sixteen adult male rabbits divided into four groups A, B, C and D. Group A, B and C were exposed to 20 ml of pyrethroid insecticides containing 0.05% pralletrin and 0.15% cyfluthrin by inhalation for 10 mins, 20 mins, and 30mins respectively. Group D served as the control and was not exposed. Baseline study was done on all the animals before grouping for exposure. The pyrethroid insecticides was soaked in cotton wool (2.5g) in a container that was able to prevent the animal from ingesting it, which was placed inside&nbsp; the room A, B and C for 10 mins, 20 mins and 30 mins respectively. The rabbits were exposed for three weeks and samples were collected at the end of each week. Exposure was discontinued after day 21 and Samples were collected again on day 28 and 35 respectively which is the fourth and fifth week. All the animals were monitored twice daily for clinical signs like jerky movement, skin scratching, licking of legs and other body parts.</p> <p><strong>Results:</strong> Data analysis revealed that there was significant effect of inhalation of insecticides on coagulation profile of rabbits at 10 min, 20 mins and 30 mins of exposure. There was significant decreased in PTTK and INR values. But, there was no significant effect on prothrombin time.</p> <p><strong>Conclusion: </strong>The results from this study have shown that aerosol of these pyrethroid insecticides (pralletrin and cyfluthrin) has effect on coagulation parameters. It is thus recommended that one should avoid exposure to the aerosol of these insecticides during domestic, veterinary,agricultural or industrial use.</p> Emily Funmilayo Eyitayo, Olusegun Taiwo Oke, Dorcas Yetunde Obazee, Emmanuel Ifeanyi Obeagu ##submission.copyrightStatement## https://journalahrj.com/index.php/AHRJ/article/view/30204 Tue, 13 Sep 2022 00:00:00 +0000 Significance of Some Hematological Parameters in Type 2 Diabetic Patients in the Enugu State University of Science and Technology Teaching Hospital, Enugu State, Nigeria https://journalahrj.com/index.php/AHRJ/article/view/30205 <p><strong>Background: </strong>Diabetes mellitus has over the years become a public health challenge and a complex disease which is characterized by chronic hyperglycemia that results in microvascular and macrovascular complications.</p> <p><strong>Aim: </strong>The present study was designed to determine the hematological profile of type 2 diabetic (T2DM) patients in Enugu State University of Science and Technology Teaching Hospital, Enugu State, Nigeria.</p> <p><strong>Methods: </strong>A total of 240 subjects comprising 120 T2DM (60 males and 60 females) aged 20-55 years and 120 apparently healthy age and gender-matched controls were recruited for the study.&nbsp; Blood sample (5.0ml) was collected from each subject for the analyses of the hematological parameters using Mindray 530 BC automated analyzer, Mindray Japan. The data was analysed using T-test and level of significance set at p&lt;0.05.</p> <p><strong>Results:</strong> The result revealed significantly decreased Mean Cell Volume (MCV) (86.54 <u>+</u> 0.85) in the T2DM subjects compared to the controls (89.88 <u>+</u> 1.09) and significantly increased Basophil count (0.93 <u>+</u> 0.09) in the T2DM subjects compared to the controls (0.22 <u>+</u> 0.03).</p> <p><strong>Conclusion:</strong> These findings supports the claim for alterations in hematological profile of T2DM patients.</p> Alphonsus Ogbonna Ogbuabor, Juliet Ada Chineke, Silas Anayo Ufelle ##submission.copyrightStatement## https://journalahrj.com/index.php/AHRJ/article/view/30205 Thu, 15 Sep 2022 00:00:00 +0000 Cytogenetic Profile of Chronic Myeloid Leukaemia (CML) in Patients Received at the Medical Biology Laboratory of the Pasteur Institute in Dakar https://journalahrj.com/index.php/AHRJ/article/view/30206 <p><strong>Background:</strong> The objective of this study was to determinate cytogenetic profiles of patients received at the medical biology laboratory of the Pasteur Institute of Dakar over a 2-year period.</p> <p><strong>Methods:</strong> Twenty-nine samples on heparinized tube from patients suspected of having myeloproliferative syndromes with clinical information sheets were sent to the Biomnis laboratory for cytogenetic study. Karyotyping and molecular cytogenetic analysis: fluorescence in situ hybridization (FISH) was performed on all patients.</p> <p><strong>Results:</strong> The mean age of the patients was 39 years [CI=95%] with extremes of 15 and 68 years. There was a male predominance with a gender ratio M/W of 1.41 and the most represented age group was 35-49 years. The molecular study showed the BCR-ABL transcript present in 86% (n=25) of patients and absent in 14% (n=4). In conclusion 82.76% (n=24) of the patients had chronic myeloid leukaemia with the transcript present in 100% including one patient with translocation t(9;22;16) (q34;q11;p13) as an associated chromosomal abnormality, one case of Phi+ acute leukaemia was also noted.</p> <p><strong>Conclusion:</strong> The FISH analysis is a significant technological advance in the diagnosis and monitoring of CML treatment in Senegal because it can detect several variants resulting from the t(9;22) translocation, which would allow the prediction of primary or secondary resistance in patients.</p> Abdou Diop, Thierno Abdoulaye Diallo, Babacar Ndiaye, Abdoulaye Seck, Chantal Mahou, Phillippe Dubrous ##submission.copyrightStatement## https://journalahrj.com/index.php/AHRJ/article/view/30206 Thu, 29 Sep 2022 00:00:00 +0000