Asian Hematology Research Journal

Correlation of Mean Platelet Volume (MPV) and Platelet Count in Dengue Cases as Prognostic Markers

Javid Ashraf Wani, Tejashwini Huchannavar, H. P. Shruthi, Prof Rajesh Shenoy — Wed, 08 Apr 2026 00:00:00 +0000

Background: Dengue is rapidly emerging arboviral disease associated with considerable morbidity, mainly due to thrombocytopenia and hemorrhagic complication. Mean platelet volume (MPV) which reflects platelet production and activation, may act as a useful prognostic indicator in dengue infection.

Aim: The present study determines the relationship between MPV and platelet count in dengue patients and evaluate its prognostic vale.

Material and Methods: This cross -sectional study included 62 serologically confirmed dengue patient’s admitted between June and September 2022. Complete blood count parameter were measured using an automated hematology analyzer. Statistical analysis was performed using IBM SPSS version 25. Since data were non-normally distributed (Kolmogorov–Smirnov test), Spearman correlation was applied. A p-value <0.05 was considered statistically significant.

Results: The median platelet count was 1.87 lakhs per microliter (IQR 1.18) and median MPV was 9.64 fL (IQR 1.31). A statistically significant correlation was observed between platelet count and MPV (r = 0.65, p <0.0001). Similar significant correlations were observed across all age groups.

Conclusion: Regular monitoring of MPV along with platelet count may serve as a useful prognostic tool for predicting significant thrombocytopenia in dengue patients. MPV may assist in the early detection of patients who are at risk of developing complications.

Evaluation of P-Selectin Level in Sickle Cell Disease Patients with Leg Ulcers in South-South Nigeria

Fri, 06 Mar 2026 00:00:00 +0000

Background: Sickle cell leg ulcers (SCLU) contribute to significant morbidity in patients with sickle cell disease (SCD). Nigeria has the highest burden of sickle cell disease (SCD) and about one-third of the patients have leg ulcers. Although several factors contribute to the development of leg ulcer, the role of P-selectin has not been adequately explored globally.

Aim: This study aimed at determining the level of P-Selectin in SCD patients with leg ulcers in Benin City, Nigeria.

Methods: This was a hospital based comparative study conducted at the University of Benin Teaching hospital (UBTH) between June 2023 and November 2023 among SCD patients and healthy controls. Eighty-eight subjects including 33 SCD patients with leg ulcer, 33 SCD patients without leg ulcers and 22 HbAA controls were recruited. Data were analyzed using the Statistical Package for Social Sciences (SPSS) version 23.

Results: The mean age (SD) of SCD patients with leg ulcers, SCD controls and HbAA controls were 29±6.6yrs, 29.3±5.9yrs and 29.9±6.7yrs respectively. The differences in mean age across the study groups were not statistically significant (p=0.932). Nineteen (57.6%) SCD individuals with leg ulcers were females and fourteen (42.4%) were males. There was no statistically significant difference in the sex distribution between the case group and controls (p=0.521). The median P-selectin level was lower in SCD patients with leg ulcers compared with SCD patients without leg ulcers (0.21pg/ml vs 0.36pg/ml), while the levels were higher than their age and sex matched HbAA controls (0.21pg/ml vs 0.16pg/ml). The differences were not statistically significant (p=0.063). The study did not find any significant association between the level of p-selectin and the severity of leg ulcer (p=0.442).

Conclusion: There was no significant association between P-selectin level and SCLU in our study population.

Comparison of Whole Blood Clotting Time and Platelets Count among Different Hemoglobin Variants among Students in A Tertiary Institution in Nigeria

Eledo, Benjamin Onyema — Mon, 16 Mar 2026 00:00:00 +0000

Blood coagulation is a complex physiological process that prevents excessive haemorrhage and preserves the integrity of the circulatory system. It comprises a sequence of tightly regulated events that culminate in the formation of a blood clot, or thrombus, at the site of vascular injury. whole blood clotting time and platelets count provide valuable information about the coagulation system's functionality, aid in diagnosing various hematological disorders, guide treatment decisions, and help assess bleeding and clotting tendencies in individuals This study compared whole blood clotting time and platelets count among different hemoglobin variants among students of Madonna University Nigeria, Elele. A total of two hundred and fifty three apparently healthy subjects comprising132 hemoglobin AA subjects and 121 hemoglobin AS subjects within the age range of 17 to 35 years were enrolled for this study. A total of 141 were males while 112 were females. Before the commencement of the study, ethical approval was obtained from the university as well as informed oral consent of all subjects. Blood samples were collected using standard venipuncture technique and analyzed for whole blood clotting time and platelet count using Lee and White method and method described by Cheesbrough, 2006 respectively. The result obtained for platelets count from subjects with hemoglobin AA variants (177.2±17.4 x10⁹) were higher than subjects with hemoglobin AS variants (175.8±19.7 x10⁹) but statistically non-significant (p>0.05). The result obtained from subjects with hemoglobin AA variants and subjects with hemoglobin AS variants showed a non-significant increase (p>0.05) in whole blood clotting time. Subjects with hemoglobin AA variants with (4.94±0.84 minutes) when compared to subjects with hemoglobin AS variants (4.83±0.98 minutes. When the results were compared between the male and female subjects, it showed a significant decrease (p<0.05) in platelets count of males (167.9 ± 17.8) when compared to females (180.5 ± 2.9). But, there was a significant increase (p<0.05) in clotting time of males (5.4 ± 1.1) when compared to females (4.7 ± 0.7)). In conclusion, this research work demonstrates that whole blood clotting time remains largely uniform irrespective of hemoglobin type. However, notable disparities in platelet counts among the different hemoglobin variants were observed, emphasizing the potential influence of these genetic variations on this parameter.

A Cross-Sectional Study on Clinical and Laboratory Profile of Patients with Thrombocytopenia in a Tertiary Care Teaching Hospital

C Ramachandra Bhat, Chinta Vempa Reddy — Sat, 21 Mar 2026 00:00:00 +0000

Background: Thrombocytopenia is a common haematological abnormality encountered in clinical practice and is associated with a wide spectrum of etiologies ranging from transient infections to chronic systemic disorders. The clinical significance of thrombocytopenia depends not only on platelet count but also on the underlying cause and platelet functional parameters.

Aim: The present study evaluates the clinical and laboratory profile of patients with thrombocytopenia and assesses the association between platelet count, platelet indices, and bleeding manifestations.

Materials and Methods: This hospital-based cross-sectional observational study was conducted in the Department of General Medicine at a tertiary care teaching hospital over a period of 18 months. A total of 110 adult patients with platelet counts less than 100,000/µL were included. Detailed demographic and clinical data were recorded. Laboratory evaluation included complete blood count with platelet indices—mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit (PCT), and platelet large cell ratio (P-LCR). Statistical analysis was carried out using SPSS software. Associations between categorical variables were assessed using the Chi-square test, while the comparison of mean platelet indices between groups was performed using an independent t-test. A p-value <0.05 was considered statistically significant.

Results: The majority of patients were aged 31–45 years with a male predominance (61.8%, p=0.013). Fever was the most common presenting symptom (70.9%, p<0.001), and bleeding manifestations were observed in 34.5% of patients. Dengue fever was the leading cause of thrombocytopenia (35.5%, p<0.001). A statistically significant association was found between bleeding manifestations and severe thrombocytopenia (<20,000/µL), with 85.7% of these patients experiencing bleeding compared to only 15.4% in those with counts >50,000/µL (p<0.001). Furthermore, patients with bleeding had significantly higher MPV (12.1±1.3 vs. 10.7±1.2 fL, p<0.01) and PDW (18.4±2.1% vs. 15.9±2.0%, p<0.01), and lower PCT (0.09±0.03% vs. 0.13±0.04%, p<0.05) compared to those without bleeding.

Conclusion: Thrombocytopenia is predominantly caused by infectious etiologies in tropical regions. The statistical analysis confirms that while severe thrombocytopenia is strongly associated with bleeding risk, platelet indices—specifically MPV, PDW, and PCT—serve as valuable independent adjunctive markers for assessing bleeding risk and understanding underlying mechanisms, aiding in improved clinical evaluation and management.

Evaluation of ADAMTS13 Activity and von Willebrand Factor Antigen Levels in Patients with Sickle Cell Anaemia

Wed, 25 Mar 2026 00:00:00 +0000

Background: Sickle cell anaemia (SCA) is characterised by chronic haemolysis, inflammation, and recurrent vaso-occlusive crises, with endothelial dysfunction playing a central role in disease pathophysiology. von Willebrand factor antigen (vWF:Ag) and ADAMTS13 are key regulators of haemostasis, and their imbalance may contribute to microvascular thrombosis in SCA

Aim: This study aimed to evaluate ADAMTS13 activity and von Willebrand factor antigen levels in patients with sickle cell anaemia.

Method: This case–control study evaluated ADAMTS13 activity and vWF:Ag antigen levels among patients with sickle cell anaemia attending selected health centers in Ado-Ekiti, Ekiti State, Nigeria. A total of 60 participants were recruited, comprising 30 patients with sickle cell anaemia and 30 apparently healthy individuals who served as controls. The sickle cell patients included 16 patients in steady state and 14 patients experiencing sickle cell anaemic crisis. Venous blood samples were collected from all participants for the determination of ADAMTS13 activity and Vwf antigen levels using enzyme-linked immunosorbent assay (ELISA). Full blood counts were also performed using a Sysmex haematology Optimiza. Data analysis was carried out using the Statistical Package for Social Sciences (SPSS) version 26.0. Results were expressed as mean ± standard deviation. Analysis of variance (ANOVA) was used to assess differences among the study groups, while Tukey’s post hoc multiple comparison tests were applied to identify specific group differences. Statistical significance was set at p < 0.05.

Results: The mean value of vWF:Ag of steady-state (207.64 ± 122.49 ng/mL) and crisis SCA patients (189.52 ± 100.47 ng/mL) was significantly higher compared with the control (60.92 ± 35.42 ng/mL). Steady-state patients showed significantly higher mean values than those in crisis (p < 0.001). ADAMTS13 activity was comparable across all three groups, with mean values of 0.83 ± 0.09 IU/mL in controls, 0.81 ± 0.17 IU/mL in steady-state patients, and 0.87 ± 0.14 IU/mL in crisis patients. No significant difference was observed among the groups (p = 0.428).

Conclusion: The study concludes that vWF:Ag level is a better biomarker for the severity of the inflammatory endothelial vasculopathy, enhancing thrombotic complications than ADAMTS13Monitoring vWF levels may provide valuable insight into disease activity and severity, potentially informing clinical management and risk stratification of patients with sickle cell anaemia.

Some Haematological and Coagulation Parameters Changes among Car Painters in Sokoto Metropolis

Thu, 26 Mar 2026 00:00:00 +0000

Background: The most severe health effects associated with activities in small-scale industries are often not immediately observable, but instead manifest after prolonged exposure, frequently later in the lives of affected individuals. This latency in the development of adverse outcomes complicates timely detection and intervention. One such activity is automobile painting, which is commonly undertaken in informal auto garages where occupational safety measures are often inadequate. Exposure to paint and its associated chemical constituents has been linked to a range of health conditions, among which respiratory and dermatological disorders are the most prevalent. Inhalation of paint vapours and contact with hazardous substances can lead to both acute and chronic health complications affecting the respiratory system and skin. In light of these concerns, the present study aimed to assess the effects of paint exposure on selected haematological and haemostatic parameters among car painters operating in small-scale auto garages in Sokoto metropolis.

Materials and Methods: To carry out the study, questionnaires were administered to car painters in auto garages. They were interviewed to get details of disease symptoms and other issues to support the information captured by the questionnaires. A sample population of forty six car painters from auto garages and twenty four control subjects were selected in the study area. Venous blood sample was collected and used to determine Full Blood Count (FBC), Prothrombin Time (PT) and activated Partial Thromboplastin Time (aPTT). Data were analyzed by using Statistical Package for Social Sciences (SPSS) version 25. Student T - test was used to compare Full Blood Count (FBC), aPTT and PT among car painters and control.

Results: The results obtained showed that the mean values of WBC (6.774±3.391), LYM (2.741±1.115), MXD (0.778±0.553) and NEUT (3.513±3.052) compared to the control WBC (3.800±1.132), LYM (2.054±0.616), MXD (0.321±0.172), NEUT (1.508±0.898) were significantly higher than the control (p < 0.05). The result obtained showed that the mean value of aPTT (32.39±7.987) and PT (13.39±2.285) of car painters were higher compared to control (25.96±1.829, 12.25±1.391) (p< 0.05). The study also showed that years of exposure has prolongation effect on levels of aPTT (p=0.013).

Conclusion: There is need for continuous advocacy on use of protective wears and reduction of exposure time to prevent effect of prolonged exposure on car painters.

Evaluation of the Hematopoietic Effects of Methanolic Leaf Extract of Ficus capensis in Phenylhydrazine-Induced Anemic Wistar Rats

Mon, 30 Mar 2026 00:00:00 +0000

Iron Deficiency Anemia (IDA) is a condition characterized by a decrease in the number and size of red blood cells, leading to inadequate oxygen delivery to tissues and organs. It is the most common nutritional disorder worldwide, affecting approximately 1.6 billion people globally. Caused by insufficient iron intake, absorption, or chronic blood loss . The main causes of IDA can be classified into three categories: inadequate dietary iron intake, increased iron requirements, and chronic blood loss The aim of this study was to evaluate the effects of methanolic leaves extract of Ficus capensis on some haematological parameters in phenylhydrazine-induced iron deficiency anaemic Wistar rats. This is an experimental design which comprises of 36 wistar rats with 5 rats in each group. Group 1 as Normal Control, group 2 as negative control, group 3 positive control, group 4 Low-Dose, group 5 Medium-Dose, group 6 High-Dose and group 7 extract and ferrous sulfate. In conclusion treatment with Ficus capensis extract led to a notable normalization of these leukocytic parameters. The medium dose and high dose extract groups maintained WBC counts. Treatment with F. capensis normalized platelet parameters in a dose-dependent manner. Low-, medium-, and high-dose extracts restored PLT counts to physiological ranges. Shows that the methanolic extract of Ficus capensis leaves contains a complex mixture of fatty acids, esters, terpenoids, and hydrocarbons phytochemicals typically associated with antioxidant, anti-inflammatory, antimicrobial, and hematopoietic (blood building) activities. The medium dose and high dose extract groups maintained WBC counts. In conclusion treatment with F. capensis normalized platelet parameters in a dose-dependent manner. Low-, medium-, and high-dose extracts restored PLT counts to physiological ranges. Shows that the methanolic extract of Ficus capensis leaves contains a complex mixture of fatty acids, esters, terpenoids, and hydrocarbons phytochemicals typically associated with antioxidant, anti-inflammatory, antimicrobial, and hematopoietic (blood-building) activities.

Serum Lactate Dehydrogenase and its Relationship with Lipid Profile and Fasting Blood Sugar in Patients with Circulatory System Diseases in Diwaniyah Governorate

Russel Fouad Hashim, Nabaa Mohsin Kareem, Marwa Abbas Wannas — Sat, 11 Apr 2026 00:00:00 +0000

Cardiovascular diseases (CVDs) remain the leading cause of global mortality, characterized by complex pathophysiological changes that begin with endothelial dysfunction and lipid accumulation. In this context, Lactate dehydrogenase (LDH) serves as a vital cellular biomarker released during tissue damage, offering potential for the early detection of cardiovascular injury. This analytical cross-sectional study aimed to evaluate the correlation between serum LDH levels and essential biochemical indicators (lipid profile and fasting blood sugar) among patients with circulatory system diseases in Al-Diwaniyah Governorate. The study included 75 participants: 40 diagnosed patients and 35 healthy controls. Serum LDH, fasting blood sugar (FBS), and complete lipid profiles were measured, alongside demographic data collection. Results demonstrated a highly significant elevation (p < 0.001) in serum LDH levels in the patient group (345.6 ± 42.3 U/L) compared to controls (175.4 ± 28.1 U/L). This increase was accompanied by severe biochemical dysregulation, including markedly higher FBS, total cholesterol, triglycerides, and LDL, alongside reduced HDL levels. Exacerbation of these markers was strongly associated with increased BMI and a positive family history. In conclusion, LDH is an effective biomarker for the early detection of circulatory diseases. Integrating routine LDH measurements into cardiovascular assessments can significantly enhance risk stratification, diagnostic accuracy, and early clinical management.

Prevalence of Iron Deficiency Anemia among Children in Habanyia City, Iraq

Wed, 15 Apr 2026 00:00:00 +0000

Background: Iron deficiency anemia (IDA) remains a major global public health concern, particularly affecting infants and young children. This is the first public study in Habanyai city to estimate the prevalence rate of iron deficiency anemia among children.

Objectives: To study the prevalence of iron deficiency anemia among children from 1 month to five years in Habanyia city, Iraq. Study the demographic distribution of patients and some possible related risk factors.

Material and Method: A cross-sectional community-based study done on children from one month to five years in Habanyia city in the period from the first of January 2026 to the 10 March 2026. A special well-trained staff was sent for houses belonging to all health centers of Habania sectors, blood was taken from all children who fit the criteria of this study and a complete blood picture and serum ferritin tests were done. A child was regarded as having iron deficiency anemia in this study if serum hemoglobin below 11 g/dL, MCV was below 80, and serum ferritin was below 12 μg/L. Data collected for all studied children about age, gender, maternal level of education, economic status of the family and residence.

Result: The prevalence rate of iron deficiency anemia among children was 18.4%. Boys were slightly non-significantly more prevalent than girls. The lower age group was significantly more prevalent. Low maternal educational level and low economic status of the families were recorded as risk factors, while residence was not.

Conclusion: A high prevalence rate of iron deficiency anemia was reported among children in this study. Educational sessions and TV programs for families about this problem is recommended.