Asian Hematology Research Journal

Cardio-hematoprotective Effects of Manihot esculenta and Vernonia amygdalina Leaf Extract against Phenylhydrazine-induced Hemolytic Anemia in Rats

Wed, 03 Jun 2026 00:00:00 +0000

Phenylhydrazine (PHZ)-induced hemolytic anemia is associated with oxidative stress and hematological dysfunction. This study evaluated the cardio-hematoprotective effects of combined Manihot esculenta and Vernonia amygdalina leaf extracts in PHZ-induced rats. Rats were divided into four groups: neutral control, PHZ-treated, PHZ + standard drug (Oreifer), and PHZ + plant extract. Oxidative stress biomarkers (MDA, SOD, CAT, GSH) in cardiac tissue and hematological parameters (WBC, RBC, Hb, PCV, PLT) were assessed. PHZ administration significantly increased malondialdehyde (MDA) levels while reducing antioxidant enzymes (SOD, CAT) and glutathione (GSH), indicating oxidative stress. It also caused marked reductions in red blood cells (RBC: 2.9 ± 0.82 ×10⁶/µL), hemoglobin (HGB: 31.42 ± 7.0 g/dL), and packed cell volume (PCV: 26.14 ± 5.5%), while elevating white blood cells (WBC: 13.12 ± 2.1 ×10³/µL) and platelets (PLT: 72.68 ± 12.4 ×10³/µL). Treatment with the combined plant extract significantly (p < 0.05) reduced MDA levels and restored antioxidant defenses, with notable improvements in CAT and GSH levels. Hematological parameters were also significantly improved, with restoration of RBC, Hb, and WBC levels toward normal. The effects of the extract were comparable to the standard drug in most parameters. These findings suggest that Manihot esculenta and Vernonia amygdalina leaf extracts possess potent antioxidant, cardioprotective, and hematoprotective properties, and may serve as promising therapeutic agents against oxidative stress and hemolytic anemia. Further dose and duration optimization are recommended.

Assessment of Some Haematological Parameters in Patients Infected with Hepatitis C Virus in Owerri, Nigeria

Wed, 03 Jun 2026 00:00:00 +0000

Background: Hepatitis C virus (HCV) infection is a chronic viral disease of global public health importance characterized by progressive liver injury and numerous extrahepatic manifestations. Haematological abnormalities are among the most common systemic complications and contribute significantly to disease morbidity and prognosis.

Objective: This study evaluated selected haematological parameters - packed cell volume (PCV), platelet count, total white blood cell (WBC) count, and differential WBC counts - among HCV-infected patients in Owerri, Nigeria.

Methods: A hospital-based case control study involving 60 participants aged 18–60 years was conducted. Thirty serologically confirmed HCV patients and 30 age- and sex-matched healthy controls were recruited. Blood samples were analysed using standard manual haematological techniques. Statistical analysis was performed using SPSS version 25.0 with significance set at p ≤ 0.05. Results: HCV patients had significantly reduced PCV, platelet count, WBC count and neutrophil percentage, while lymphocyte percentage was significantly higher. No significant sex-based differences were observed. Strong negative correlations existed between lymphocyte and neutrophil percentages.

Conclusion: Chronic HCV infection is associated with anaemia, thrombocytopenia, leukopenia, neutropenia and relative lymphocytosis. Routine haematological monitoring is essential in clinical management.

Acute Pediatric Immune Thrombocytopenic Purpura with Severe Thrombocytopenia: A Case Report

Rahul Shil — Mon, 25 May 2026 00:00:00 +0000

Immune thrombocytopenic purpura (ITP) is an acquired autoimmune disorder characterized by isolated thrombocytopenia and an elevated risk of bleeding. It frequently manifests in pediatric patients following viral infections. We present a case of a one-year-old female child who presented with petechial rash and gum bleeding. Laboratory investigations revealed severe thrombocytopenia. The patient was diagnosed with ITP and managed with corticosteroids and supportive care. Early diagnosis and prompt intervention resulted in clinical improvement, underscoring the significance of timely management in pediatric ITP.

Haemophilia A in Female Carriers: Diagnostic and Therapeutic Insights from Two Case Reports

Jahar Lal Baidya, Abhijit Dutta — Thu, 04 Jun 2026 00:00:00 +0000

Haemophilia A is a rare X-linked inherited bleeding disorder caused by a deficiency or a defect in coagulation factor VIII. Due to a recessive X–linked inheritance pattern, most men are affected, whereas their female relatives remain carriers. There is little experience about the rare symptomatic haemophilia carriers (1 in 100,000 women); there is no standard protocol to prevent the bleeding complications. We report two cases of first-time diagnosed haemophilia A carrier- one is a young adolescent girl, and another is an antenatal woman. Pregnancy normally induces a hypercoagulable state with increased Factor VIII, vWF, and fibrinogen. However, in haemophilia A carriers and women with von Willebrand disease, factor levels may remain low until the second trimester, increasing miscarriage risk. Effective management necessitates early recognition of the condition, the implementation of targeted haemostatic interventions, and coordinated multidisciplinary care. However, therapeutic options remain limited in resource-constrained settings, thereby posing significant challenges to optimal clinical management. In this context, strengthening clinical awareness, alongside the development and implementation of standardised management protocols, is essential to improve patient outcomes and reduce morbidity among affected women.