Open Access Case Study

Open Access Case Study

Biclonal B-Chronic Lymphocytic Leukemia: A rare Case Report with Review of Literature

Bansal Shashi, Syeda Firdos Jamil, Talwar Amandeep, Sharma Upendra

Asian Hematology Research Journal, Page 35-39

Chronic Lymphocytic Leukemia/Small Lymphocytic Leukemia is a neoplasm composed of monomorphic small mature B cells that co-express CD5, CD23 and one type of immunoglobulin light chain either kappa or lambda, which  is necessary for the diagnosis. Here, we report a case in a 65 year woman with morphological diagnosis of B-Chronic Lymphocytic Leukemia. On flowcytometry analysis, immunoglobulin light chain restriction was not apparent as B cells expressed both kappa & lambda light chains without a clear monotypic population. Sequential gating on flowcytometry revealed two monoclonal B-cell populations and helped in identification of this rare case of biclonal disease in chronic lymphocytic leukemia (CLL).

Open Access Original Research Article

Prevalence of Glutathione S Transferase (GSTM1, GSTP1 and GSTT1) Genes Polymorphisms among Pediatric Sudanese Patients with Sickle Cell Anemia

Naif Taleb Ali, Ozaz Yagoup Mohammed Ahmed, Fayad Osman Mohammed, Huda Mohammed Haroun

Asian Hematology Research Journal, Page 1-11

Background: Sickle cell disease (SCD) is taken into account as one of the foremost types of anemia in Sudan, particulary in the western part of the country. The glutathione system plays a vital role in the removal of endogenous products of peroxidation of lipids, thus protecting cells and tissue against damage from oxidative stress. Impairment of the glutathione system as result of genetic polymorphisms of glutathione S-transferase (GST) genes is anticipated to increase the severity of SCD manifestations.

Aims/Objectives: This study was aimed to evaluate the rate of GSTM1, GSTT1 and GSTP1 gene polymorphisms among sickle cell anemia pediatric patients in Sudan.

Study Design: Case control study.

Place and Duration of Study: This study carried out in Khartoum town in Jafar Ibn Auf Pediatric Hospital / Khartoum during the period (June 2017 to June 2020).

Methodology: The total subjects of the confirmed diagnosis were 126 and 126 control. Among these cases of SCA 78 (61.9%) are males and 48 (38.1%) are female and for control 80 (63.5%) are male and 46 (36.5%) are female.

We measured the frequency distribution of the three GSTs gene polymorphisms, GSTM1 and GSTT1 genotypes were determined by polymerase chain reaction (PCR). GSTP1 genotyping was conducted with a PCR-restriction fragment length polymorphism assay, SPSS version 23 was used to analyze the data.

Results: The GSTM1 null genotype frequency was found to be slightly lower in the control group, (30.2% as opposed to 33.3% in SCA patients), but this difference was not considered to be statistically significant (OR = 1.16, 95% CI: 0.68-1.97; p- value = 0.5884), GSTT1 was found in 47.6% of SCA patients and 77.8% of the Control but the frequency of individuals carrying the GSTT1 null genotype was significantly higher among SCA patients, 52.4% compared to 22.2% of the Control; (OR = 3.85, 95% CI: 2.23- 6.65; p-value =0.0001). Individuals with a combined GSTM1 null/GSTT1 null genotype had an estimated 11.7-fold increased risk of SCA (OR=11.7; CI=2.67-51.2; p-value=0.0011).

The homozygous mutant type (Val/Val) of GSTP1 showed significant difference between patients and controls (OR= 6.53, 95% CI: 1.41-30.24; P-value = 0.0164).

Conclusion: The GSTT1 polymorphism and combined form of GSTM1 null/GSTT1 null genotype and the homozygous mutant type (Val/Val) of GSTP1 increase the risk of sickle cell anemia.

Open Access Original Research Article

A Study on Serum Biochemical Analysis “Broiler” Feed Diets Containing Graded Levels of Locust Beans (Parkia biglobosa) Seed Meal in Poultry Production and Research Unit of the Department of Animal Science, Usmanu Danfodiyo University Sokoto, Nigeria

Abubakar Yusuf Kakagida, Musa Mabu Isa, Abubakar Bello Anka, Mohammed Shu’aibu Shinkafi, Audu A. Mohammed

Asian Hematology Research Journal, Page 19-28

The study was carried out evaluate the effects of feeding locust bean (Parkia biglobosa) seed at graded levels on the serum biochemistry of “broilers”. Two hundred and forty broilers were used which were randomly allocated to four treatment groups, each replicated four times in a completely randomized design. The diets consisted of 0% level of LBSM which served as experimental control, while other three diets includes; 5, 10, and 15% levels of LBSM. The experiment was divided into two phases (starter and finisher) each of which lasted for 28 days. Broiler fed 5% LBSM had higher value for packed cell volume (PCV) of 33.25% and hemoglobin concentration (HC) (11.79 g/dl) which was significantly higher (P<0.05) than the PCV and the hemoglobin concentration (HC) of the broiler fed the other diets. The values of white blood cell (WBC) for broilers fed 5% and 10% were statistically similar (13.44 and 13.26 x103/mm3) (P>0.05) but significantly higher (P<0.05) than values obtained from birds fed the Control Diet and 15% LBSM (11.54 x103/mm3) (10.21 x103/mm3). Red blood cell, (RBC) values obtained from the broilers fed the control diet, 10% and 15% LBSM (2.12, 2.43 and 2.32 respectively) 106/mm3 did not differ significantly from one another (P>0.05) but were significantly lower (P<0.05) compared to the value obtained from the birds fed 5% LBSM (3.55 x106/mm3). There was no significant difference (P>0.05) in the mean corpuscular volume (MCV) of the broilers fed the experimental diets. However, the mean corpuscular hemoglobin concentration (MCHC) values obtained from broilers fed 5% LBSM (36.100%) was higher (P<0.05) than the values obtained from birds fed 10% LBSM.

Open Access Original Research Article

Frequency of CCR5-delta32 Mutant Allele in Sudanese Patients with Sickle Cell Anemia

Tarig. A. M. Hamid, Basema. O. E. Hamza, Nasreldeen. A. M. Gaufri

Asian Hematology Research Journal, Page 29-34

Background: Sickle cell anemia (CSA) is one of a group of hemoglobin disorders known as sickle cell disease in which the sickle β-globin gene is inherited. The pathophysiology of sickle cell disease (SCD) is based on the chronic hemolysis, vaso-occlusive episodes, infection and chronic inflammatory conditions. The CCR5 gene which encodes CCR5,Th5,cell associated chemokine receptor act as pro-inflammatory mediator, the presence of mutant allele known as CCR5 delta 32 makes it non- functional, and lower inflammatory picture. Thus it could confer a selective advantage on patient with sickle cell disease because it induce less efficientTh1 response (decrease inflammation and morbidity) its effect on the inflammatory response and morbidity in patients with sickled cell disease.

Objective: This study aimed at the detection of the frequency of CCR5delta 32 polymorphism among Sudanese patients with SCA.

Materials and Methods: This is a case control study, conducted in Alneelain University –khartoum state during the period from August to December 2018. A total of study population, 60 participants (30 patients with SCA and 30 normal controls), were enrolled in this study. 2.5 milliliter of EDTA anticoagulated blood was collected from each subject. DNA was extracted by salting out method, and target DNA regions of the CCR5 delta 32 gene were amplified using allele specific polymerase chain reaction (AS-PCR).

Results: The frequency of CCR5 delta32 polymorphism in study populations was 0%.

Conclusion: There is no any CCR5 delta32 among Sudanese patients with sickle cell anemia.